Liang Qiya, Pan Zhili, Ai Zhilu, Yuan Huanhuan, Yang Yong, Xiao Yaru, Yang Jingfan, Lei Mengmeng, Huang Zhongmin
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.
Key Laboratory of Staple Grain Processing, Ministry of Agriculture and Rural Affairs, Zhengzhou 450002, China.
Food Chem X. 2025 Jul 17;29:102783. doi: 10.1016/j.fochx.2025.102783. eCollection 2025 Jul.
This study analyzed the effects of different freezing methods on the flavor characteristics of preprepared green pepper and shredded pork dishes using electronic-nose (E-nose), electronic-tongue (E-tongue), and Headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) technologies. It explored the correlation between flavor changes and freezing rate, microstructure, water distribution, and texture. Results showed significant effects of freezing methods on flavor profiles. Principal component analysis confirmed that E-nose and E-tongue effectively distinguished odor and taste characteristics, with liquid nitrogen freezing yielding sensory quality closest to fresh samples. HS-GC-IMS determined 31 key markers via Orthogonal partial least squares discriminant analysis (OPLS-DA). Correlation analysis revealed that rapid freezing shortened ice crystal formation time, preserved myofibrillar structure, and inhibited bound water migration, retaining beneficial flavors like acetic acid-D and acetic acid ethyl ester while suppressing undesirable ones. This study confirms that higher freezing rates maintain sensory quality and characteristic flavors, providing a basis for optimizing freezing processes.
本研究采用电子鼻(E-nose)、电子舌(E-tongue)和顶空气相色谱-离子迁移谱(HS-GC-IMS)技术,分析了不同冷冻方法对预制青椒肉丝菜肴风味特征的影响。探讨了风味变化与冷冻速率、微观结构、水分分布和质地之间的相关性。结果表明,冷冻方法对风味特征有显著影响。主成分分析证实,电子鼻和电子舌能有效区分气味和味道特征,液氮冷冻产生的感官品质最接近新鲜样品。HS-GC-IMS通过正交偏最小二乘法判别分析(OPLS-DA)确定了31个关键标志物。相关性分析表明,快速冷冻缩短了冰晶形成时间,保留了肌原纤维结构,抑制了结合水迁移,保留了如乙酸-D和乙酸乙酯等有益风味,同时抑制了不良风味。本研究证实,较高的冷冻速率能保持感官品质和特征风味,为优化冷冻工艺提供了依据。