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使用一种新型多位点可变数目串联重复序列分析方案探索法国大陆菌株的多样性。

Exploring the diversity of strains from mainland France using a novel multiple loci VNTR analysis scheme.

作者信息

Sallen Antinéa, Leclerc Aurélie, Paillard Sandrine, Poncet Charles, Reignault Philippe, Le Roux Anne-Claire, Cunty Amandine

机构信息

Plant Health Laboratory, ANSES, Angers, France.

inov3PT, Paris, France.

出版信息

Appl Environ Microbiol. 2025 Aug 20;91(8):e0096325. doi: 10.1128/aem.00963-25. Epub 2025 Jul 31.

Abstract

UNLABELLED

The species complex (RSSC), one of the most damaging pests worldwide, includes species , , and . Since 1990, has been reported in several European countries, including France. However, the genetic diversity of in mainland France remains poorly characterized. In this study, a novel 14-variable number of tandem repeats multilocus variable-number tandem-repeat analysis (MLVA) scheme specifically designed for IIB-1 strains of is presented. This scheme effectively differentiated 384 global strains, isolated between 1963 and 2023, into 66 distinct haplotypes. The near clonality of the IIB-1 genetic group was highlighted by the presence of a predominant clonal complex encompassing 82% of the haplotypes. The 346 strains from mainland France used in this study, isolated between 1994 and 2023, were differentiated into 46 haplotypes, which suggests that, while French IIB-1 strains have been isolated from a relatively confined geographical area over time, they still exhibit some level of genetic diversity. Moreover, this MLVA scheme enabled clear discrimination between global and French/European strains which did not cluster together, allowing the inference of potential invasion routes for certain haplotypes in Europe. This MLVA scheme is thus a valuable tool for epidemiological studies, providing deeper insights into the genetic diversity of global IIB-1 strains.

IMPORTANCE

The RSSC causes bacterial wilt on over 250 plant species, including major ones like potato, and includes species , , and , all characterized as quarantine organisms in the European Union. has been reported in several European countries over the last century with major outbreaks confirmed to be IIB-1 strains in the 1990s, and recent outbreaks of raised concerns about the emergence of additional RSSC species in Europe. Understanding the genetic diversity of RSSC strains present in mainland France, along with the structure and the dynamics of their population, is critical for maintaining effective epidemiological surveillance. In particular, MLVA is known to be a suitable molecular typing tool to trace bacterial introduction pathways, monitor pathogen spread at multiple geographical scales, and assess evolutionary potential. The insights provided by our study, with the development of a new MLVA scheme, are key to maintaining robust plant health surveillance and implementing targeted, evidence-based management strategies.

摘要

未标注

种复合体(RSSC)是全球最具破坏性的害虫之一,包括种、种和种。自1990年以来,在包括法国在内的几个欧洲国家都有关于种的报道。然而,法国本土种的遗传多样性仍未得到充分描述。在本研究中,提出了一种专门为种的IIB - 1菌株设计的新型14个串联重复序列可变数目的多位点可变数目串联重复序列分析(MLVA)方案。该方案有效地将1963年至2023年间分离的384株全球菌株分为66个不同的单倍型。一个包含82%单倍型的主要克隆复合体的存在突出了IIB - 1基因组的近乎克隆性。本研究中使用的来自法国本土的346株菌株,于1994年至2023年间分离,被分为46个单倍型,这表明,尽管法国的IIB - 1菌株长期以来是从相对有限的地理区域分离出来的,但它们仍表现出一定程度的遗传多样性。此外,这种MLVA方案能够清晰地区分全球菌株与未聚类在一起的法国/欧洲菌株,从而推断出某些单倍型在欧洲的潜在入侵途径。因此,这种MLVA方案是流行病学研究的一个有价值的工具,能更深入地了解全球IIB - 1菌株的遗传多样性。

重要性

RSSC可导致250多种植物发生青枯病,包括马铃薯等主要作物,并且包括种、种和种,所有这些在欧盟都被列为检疫性生物。上个世纪在几个欧洲国家都有关于种的报道,20世纪90年代确认主要爆发菌株为IIB - 1菌株,最近种的爆发引发了对欧洲出现更多RSSC物种的担忧。了解法国本土存在的RSSC菌株的遗传多样性及其种群结构和动态,对于维持有效的流行病学监测至关重要。特别是,MLVA已知是一种合适的分子分型工具,可以追踪细菌引入途径、监测病原体在多个地理尺度上的传播,并评估进化潜力。我们的研究通过开发新的MLVA方案提供的见解,是维持强大的植物健康监测和实施有针对性的、基于证据的管理策略的关键。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42b2/12366337/3faa1f0aaed0/aem.00963-25.f001.jpg

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