Mao Xuewei, Cao Tangbo, Chen Liting, Li Min, Zhao Xingchen, Liu Hao, Duan Yun, Wang Li, Zhou Lin
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, Zhengzhou 450046, China; Henan Research Center of Green Pesticide Engineering and Technology, Henan Agricultural University, Zhengzhou 450046, China; Postdoctoral station of Crop science, Henan Agricultural University, Zhengzhou, 450046, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Pestic Biochem Physiol. 2025 Sep;213:106548. doi: 10.1016/j.pestbp.2025.106548. Epub 2025 Jul 8.
Peanut root rot (PRR) is caused by Fusarium solani, which severely affects the yield and quality of peanuts. Prochloraz is a triazole-based demethylation inhibitor (DMIs) fungicide with antifungal activity against Fusarium spp. However, the resistance risk and resistance mechanisms of F. solani to prochloraz remain poorly understood. In the current study, the EC values of 98 F. solani isolates to prochloraz ranged from 0.029 to 0.40 μg/mL, with a mean of 0.14 ± 0.07 μg/mL, and the sensitivity exhibited a unimodal distribution. Seven prochloraz-resistant (ProR) mutants were obtained through fungicide adaptation, and the resistance factor (RF) ranged from 5.81 to 83.33. The mutants demonstrated reduced fitness in mycelial dry weight and asexual reproduction. No cross-resistance was observed between prochloraz and other fungicides, including tebuconazole, pyraclostrobin, and fluazinam. Overexpression of FsCYP51A, FsCYP51B, FsMFS1, and FsMFS2 genes was detected in the resistant mutants. These results demonstrate that the risk of F. solani developing resistance to prochloraz is relatively low, and the overexpression of FsCYP51 and FsMFS may contribute to prochloraz resistance in F. solani. This study provides essential data for the resistance monitoring and resistance management strategies for prochloraz.
花生根腐病（PRR）由茄形镰刀菌引起，严重影响花生的产量和品质。咪鲜胺是一种基于三唑的脱甲基抑制剂（DMIs）类杀菌剂，对镰刀菌属具有抗真菌活性。然而，茄形镰刀菌对咪鲜胺的抗药性风险和抗药机制仍知之甚少。在本研究中，98株茄形镰刀菌分离株对咪鲜胺的EC值范围为0.029至0.40μg/mL，平均值为0.14±0.07μg/mL，敏感性呈单峰分布。通过杀菌剂驯化获得了7个抗咪鲜胺（ProR）突变体，抗药因子（RF）范围为5.81至83.33。这些突变体在菌丝干重和无性繁殖方面表现出适应性降低。在咪鲜胺与其他杀菌剂（包括戊唑醇、吡唑醚菌酯和氟啶胺）之间未观察到交叉抗药性。在抗性突变体中检测到FsCYP51A、FsCYP51B、FsMFS1和FsMFS2基因的过表达。这些结果表明，茄形镰刀菌对咪鲜胺产生抗药性的风险相对较低，FsCYP51和FsMFS的过表达可能导致茄形镰刀菌对咪鲜胺产生抗药性。本研究为咪鲜胺的抗药性监测和抗药性管理策略提供了重要数据。
