Matsuya Haruki, Maeda Mayumi, Maehashi Kenji, Yoshikawa Jun
Department of Fermentation Science and Technology, Graduate School of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-Ku, Tokyo, 156-8502, Japan.
Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-Ku, Tokyo, 156-8502, Japan.
Curr Microbiol. 2025 Aug 1;82(9):427. doi: 10.1007/s00284-025-04392-x.
In this study, we aimed to develop isomaltooligosaccharides (IMO) production as a novel industrial application of Zalaria sp. Him3, fructooligosaccharides (FOS)-producing yeast. A utilization test of carbon sources by Zalaria sp. Him3 was performed using API 50CH. Subsequently, the strain was cultivated in maltose medium, and the culture supernatant was used as α-glucosidase (AGase). AGase activity was evaluated by determining the amount of p-nitrophenol (PNP) derived from PNP-α-1,4-glucoside. IMO production was measured at 30 °C in the reaction mixture containing 280 g/L maltose and 0.25 U/mL AGase. Zalaria sp. Him3 highly utilized glucose, mannose, maltose, sucrose, and trehalose. When the strain was incubated with 150 g/L maltose for 48 h, IMO (isomaltose, panose, and isomaltotriose) were confirmed in the culture supernatant and AGase activity was 0.0165 U/mL. During IMO production using AGase in the culture supernatant, approximately 98% of initial maltose (279 g/L) was degraded for 72 h. The maximum IMO concentration was 138 g/L after 12 h of reaction. Thus, the yield of IMO production by AGase was 49.5% of the initial maltose. Zalaria sp. Him3, which is the FOS-producing yeast, can also produce IMO and serves as an industrially promising enzyme resource for the production of multiple types of oligosaccharides.
在本研究中，我们旨在开发低聚异麦芽糖（IMO）的生产，将其作为产低聚果糖（FOS）的酵母Zalaria sp. Him3的一种新型工业应用。使用API 50CH对Zalaria sp. Him3进行了碳源利用测试。随后，将该菌株在麦芽糖培养基中培养，培养上清液用作α-葡萄糖苷酶（AGase）。通过测定源自对硝基苯酚-α-1,4-葡萄糖苷的对硝基苯酚（PNP）量来评估AGase活性。在含有280 g/L麦芽糖和0.25 U/mL AGase的反应混合物中于30℃下测定IMO产量。Zalaria sp. Him3高度利用葡萄糖、甘露糖、麦芽糖、蔗糖和海藻糖。当该菌株与150 g/L麦芽糖一起孵育48小时时，在培养上清液中确认了IMO（异麦芽糖、潘糖和异麦芽三糖），且AGase活性为0.0165 U/mL。在使用培养上清液中的AGase生产IMO的过程中，约98%的初始麦芽糖（279 g/L）在72小时内被降解。反应12小时后，IMO的最大浓度为138 g/L。因此，AGase生产IMO的产率为初始麦芽糖的49.5%。产FOS的酵母Zalaria sp. Him3也能生产IMO，并且是用于生产多种类型低聚糖的具有工业前景的酶资源。
