Qi He, Wang Wenjiong, Zhang Xianghan, Shang Bingyang
Innovation Practice Platform for College Student, College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China.
Int J Anal Chem. 2025 Jul 28;2025:7941435. doi: 10.1155/ianc/7941435. eCollection 2025.
Based on the establishment and validation of a UPLC-MS/MS method for detecting glipizide in beagle plasma, the herb-drug interaction (HDI) between berberine and glipizide was studied. After gradient elution separation of glipizide and internal standard, multiple reaction monitoring was used for detection in positive ion mode. The ion reactions used for quantitative analysis were glipizide m/z 446.0 ⟶ 321.0 and IS m/z 307.1 ⟶ 220.0. Six beagle dogs were treated with glipizide alone and berberine intervention, and the pharmacokinetic changes of glipizide were compared. The UPLC-MS/MS method has good linearity and the advantages of being green, simple, sensitive, and fast. After continuous administration of berberine to beagle dogs for 7 days, the pharmacokinetic process of glipizide changed with , AUC and AUC increasing, t prolonging, and CL and Vd decreasing. When using combination therapy, attention should be paid to possible HDI.
基于建立和验证用于检测比格犬血浆中格列吡嗪的超高效液相色谱-串联质谱（UPLC-MS/MS）方法，研究了黄连素与格列吡嗪之间的药-药相互作用（HDI）。对格列吡嗪和内标进行梯度洗脱分离后，采用多反应监测在正离子模式下进行检测。用于定量分析的离子反应为格列吡嗪m/z 446.0→321.0和内标m/z 307.1→220.0。对6只比格犬分别进行单独给予格列吡嗪和黄连素干预处理，并比较格列吡嗪的药代动力学变化。该UPLC-MS/MS方法具有良好的线性，且具有绿色、简便、灵敏和快速的优点。对比格犬连续7天给予黄连素后，格列吡嗪的药代动力学过程发生改变， 、AUC和AUC增加，t延长，CL和Vd降低。联合治疗时应注意可能存在的药-药相互作用。
