Odeyemi Inioluwa Ayooluwa, Oyediran Jesutomi Aduragbemi, Ademiluyi Adedayo Oluwaseun, Oboh Ganiyu, Ogunsuyi Opeyemi Babatunde
Drosophila Research Laboratory, Functional Foods and Nutraceuticals Unit, Federal University of Technology Akure, Akure, Ondo, Nigeria.
Science Laboratory Technology Department, Federal Polytechnic Ile- Oluji, Ile-Oluji, Ondo, Nigeria.
Biometals. 2025 Aug 5. doi: 10.1007/s10534-025-00733-2.
Selenium biofortification of staple foods is a strategy for ameliorating Selenium deficiency among populations. The aim of this study is to investigate the effect of Selenium biofortification on some phytochemicals present in Gboma (Solanum macrocarpon L.) leaves and to assess the effect of its dietary inclusions on some antioxidant molecules, immunoglobulins and inflammatory cytokines in the serum of Wistar rats. Gboma, also called African Eggplant was cultivated on soil enriched with Selenium biofortified fertilizer and harvested at maturity. The phytochemicals present were assessed via HPLC. The samples were also included into the diet of Wistar rats (4% and 8% inclusions) for fourteen days. The serum was then collected and assayed for glutathione peroxidase (GPx), glutathione-S-transferase (GST) and catalase activities. Imuunoglobulins M, G, A and E as well as the inflammatory makers-tumor necrosis factor-α and interleukin-10 were also assayed for. The results showed that Selenium bioaccumulation increased the concentration of some phytochemicals such as chlorogenic acid, 4- hydroxybenzoic acid and delphinidin. An increase in the activity of the antioxidant enzymes was also observed in rats fed with Selenium biofortified Gboma leaves (4% and 8%), compared to control and rats fed diets supplemented with Gboma leaves without Selenium biofortification. In addition, IgG, IgM and IgA levels increased in the serum of treated rats, coupled with an increase in IL-10 levels. This study therefore, shows that Selenium biofortification enhanced the antioxidant and immunomodulatory properties of Gboma leaves.
主食的硒生物强化是改善人群硒缺乏状况的一项策略。本研究旨在调查硒生物强化对Gboma（大果茄）叶片中某些植物化学物质的影响，并评估其在日粮中的添加对Wistar大鼠血清中某些抗氧化分子、免疫球蛋白和炎性细胞因子的影响。Gboma，也被称为非洲茄子，种植在富含硒生物强化肥料的土壤上，成熟时收获。通过高效液相色谱法评估其中存在的植物化学物质。这些样品还以4%和8%的添加量加入到Wistar大鼠的日粮中，持续14天。然后收集血清，检测谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）和过氧化氢酶的活性。还检测了免疫球蛋白M、G、A和E以及炎性标志物——肿瘤坏死因子-α和白细胞介素-10。结果表明，硒生物累积增加了一些植物化学物质的浓度，如绿原酸、4-羟基苯甲酸和飞燕草素。与对照组以及喂食未进行硒生物强化的Gboma叶片日粮的大鼠相比，喂食硒生物强化Gboma叶片（4%和8%）的大鼠抗氧化酶活性也有所增加。此外，处理组大鼠血清中的IgG、IgM和IgA水平升高，同时IL-10水平也升高。因此，本研究表明，硒生物强化增强了Gboma叶片的抗氧化和免疫调节特性。
