用于原发性免疫性血小板减少症诊断的单克隆抗体免疫固定血小板抗原检测法（MAIPA法）与PakAuto检测法的对比分析
Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia.
作者信息
Chen Lixuan, Chen Chaolin, Tuo Rui, Ye Limei, Ouyang Shuting, Su Hao, Lai Jianxi, Feng Ying, Xiao Yang
机构信息
Department of Hematology, Shenzhen Qianhai Shekou Free Trade Zone Hospital, No 128 Shekou Industrial Seventh Road, Nanshan District, Shenzhen, 518067, Guangdong Province, China.
Hemostasis & Thrombus Lab, Guangzhou Zhili Medicine, No.11 Nanyun Fifth Road, Huangpu District, Guangzhou, 510700, Guangdong Province, China.
出版信息
Clin Exp Med. 2025 Aug 7;25(1):282. doi: 10.1007/s10238-025-01831-8.
The detection of platelet-specific antibodies plays an important role in the clinical diagnosis of primary immune thrombocytopenia (ITP). MAIPA assay and PakAuto assay are the two main methods for this purpose. However, a comprehensive comparative evaluation of these techniques in clinical practice remains lacking. In this study, we collected 48 patients with thrombocytopenia. MAIPA and PakAuto are used to examine platelet-specific antibodies synchronously. Based on methodologies and analysis of experimental results, we conclude that PakAuto assay with a high sensitivity and accuracy can offer higher throughput and broader human platelet antibodies detection capabilities, making it more suitable for screening.
血小板特异性抗体的检测在原发性免疫性血小板减少症（ITP）的临床诊断中起着重要作用。单克隆抗体固相血小板抗原分析法（MAIPA）和血小板自动化检测法（PakAuto）是用于此目的的两种主要方法。然而，在临床实践中对这些技术仍缺乏全面的比较评估。在本研究中，我们收集了48例血小板减少症患者。同时使用MAIPA和PakAuto检测血小板特异性抗体。基于方法学和实验结果分析，我们得出结论，具有高灵敏度和准确性的PakAuto检测法能够提供更高的通量和更广泛的人血小板抗体检测能力，使其更适合用于筛查。
Zotero 插件