Ijaz Farah, Ali Shaukat, Pervaiz Asim, Summer Muhammad
Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, 54000, Pakistan.
Human Genetics and Molecular Biology Department, University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, 54600, Pakistan.
Med Oncol. 2025 Aug 9;42(9):423. doi: 10.1007/s12032-025-02974-0.
Sericin is a globular protein known to have antioxidant potential due to presence of amino, carboxyl and hydroxyl groups in its structure. This study was designed to investigate the antiproliferative and apoptotic potential of sericin and sericin chitosan conjugated silver nanoparticles against colorectal cancer cells. To investigate the antiproliferative and apoptotic activity of sericin and sericin chitosan conjugated silver nanoparticles (SChiAgNPs), three human colorectal cancer cell lines (SW480, SW620, HCT116) were used. Sericin was isolated by the degumming process followed by the characterization by using FTIR, UV, XRD, and SEM techniques to confirm the isolation process and successful synthesis of SChiAgNPs. MTT assay was carried out to analyze the antiproliferative activities, while expression profiling of the genes i.e., GADD45A, BCL2, and TNF was assessed by qRT-PCR analysis. Sericin (S-Ext) and SChiAgNPs showed significant antiproliferative activities in SW480, SW620 and HCT 116 cells. Overall, there was 29-34 inhibition of viability for sericin S-Ext and 35-43 for SChiAgNPs in the three cell lines in comparison to untreated control. Expression profiling indicated the significant stimulation of GADD45A, BCL-2 and TNF genes expression in SW480, SW620 and HCT 116 cells. The GADD 45 A showed induction by 1.43-1.71-fold in SW480, 1.09-1.56-fold in SW620 and 1.25-4.55-fold in HCT 116 cells in response to treatment groups. The BCL2 showed the induction by 1.35-2.53, 1.38-3.1, and 2.32-3.76-fold in SW480, SW620, and HCT116 cells, respectively. TNF was induced by a factor of 3.9-6.43, 2.53-5.41, and 2.7-5.31-fold in in SW480, SW620, and HCT116 cells, respectively, after the exposure with compounds. Sericin and S-ChiAgNPs, showed significant growth inhibition and gene expression profiling modifications in the colorectal cancer cells. The findings provide evidence about sericin and its nanoparticle conjugates as potential anticancer medicine for colorectal cancer.
丝胶蛋白是一种球状蛋白质，因其结构中存在氨基、羧基和羟基而具有抗氧化潜力。本研究旨在探讨丝胶蛋白及丝胶蛋白-壳聚糖共轭银纳米颗粒对结肠癌细胞的抗增殖和凋亡潜力。为了研究丝胶蛋白及丝胶蛋白-壳聚糖共轭银纳米颗粒（SChiAgNPs）的抗增殖和凋亡活性，使用了三种人类结肠癌细胞系（SW480、SW620、HCT116）。通过脱胶工艺分离丝胶蛋白，随后使用傅里叶变换红外光谱（FTIR）、紫外光谱（UV）、X射线衍射（XRD）和扫描电子显微镜（SEM）技术进行表征，以确认分离过程及SChiAgNPs的成功合成。进行MTT试验以分析抗增殖活性，同时通过定量逆转录聚合酶链反应（qRT-PCR）分析评估GADD45A、BCL2和TNF等基因的表达谱。丝胶蛋白（S-Ext）和SChiAgNPs在SW480、SW620和HCT 116细胞中显示出显著的抗增殖活性。总体而言，与未处理的对照组相比，丝胶蛋白S-Ext在三种细胞系中的活力抑制率为29%-34%，SChiAgNPs为35%-43%。表达谱分析表明，SW480、SW620和HCT 116细胞中GADD45A、BCL-2和TNF基因的表达受到显著刺激。在处理组的作用下，SW480细胞中GADD 45 A的诱导倍数为1.43-1.71倍，SW620细胞中为1.09-1.56倍，HCT 116细胞中为1.25-4.55倍。BCL2在SW480、SW620和HCT116细胞中的诱导倍数分别为1.35-2.53、1.38-3.1和2.32-3.76倍。在接触化合物后，SW480、SW620和HCT116细胞中TNF的诱导倍数分别为3.9-6.43、2.53-5.41和2.7-5.31倍。丝胶蛋白和S-ChiAgNPs在结肠癌细胞中显示出显著的生长抑制和基因表达谱改变。这些发现为丝胶蛋白及其纳米颗粒共轭物作为结肠直肠癌的潜在抗癌药物提供了证据。
