Kumari Aparna, Verma Barkha, Chattopadhyay Soham
Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Jharkhand, 835215, India.
Curr Microbiol. 2025 Aug 10;82(9):445. doi: 10.1007/s00284-025-04441-5.
In the present research, we isolate and characterize the thermophilic biofilm-forming bacteria of the textile industry that are responsible for the degradation of azo dye. The thermophilic bacteria that form biofilm grow at a temperature range of 45-80 ℃ and play an important role in bioremediation. Thermo-stable biofilm-producing bacteria are a unique class of microorganisms that flourish well in extreme environments. The study aims to screen and characterize thermophilic biofilm-producing bacteria isolated from the soil of the textile industry in Hindpiri, Ranchi, Jharkhand, India, to uncover their potential for Congo red decolorization. Two isolates, A1 and A2, which are able to form biofilm when grown in TSA, were used for further studies. After 3 days of incubation, maximum biofilm developed at 45 °C with pH 7-9. Biofilms were grown on glass coverslips and incubated with Congo red dye to evaluate their dye decolorization ability. Isolate A1 exhibited a high decolorization potential, 80% Congo red dye in 18 h, whereas isolate A2 had a lower efficiency, decolorizing only 60% of the dye under 45 ℃ at static conditions. 16S rRNA-based molecular characterization identified isolate A1 as Bacillus licheniformis A1 (PV362359). Analytical techniques like FESEM and FTIR analysis showed significant changes in bacterial cells and in functional groups, with a marked reduction or disappearance of peaks, respectively. Peaks at 1745 cm, 2859 cm, 2931 cm, and 1154 cm give the alterations in protein and polysaccharides associated functional groups. These demonstrate the breakdown of the azo linkages and sulfonate groups. The present study indicates the potential of thermophilic biofilm-producing bacteria for azo-dye bioremediation.
在本研究中，我们分离并鉴定了纺织工业中负责偶氮染料降解的嗜热生物膜形成细菌。形成生物膜的嗜热细菌在45-80℃的温度范围内生长，在生物修复中发挥着重要作用。产生热稳定生物膜的细菌是一类独特的微生物，能在极端环境中良好生长。本研究旨在筛选和鉴定从印度贾坎德邦兰契辛德皮里纺织工业土壤中分离出的嗜热生物膜产生细菌，以揭示它们对刚果红脱色的潜力。两个分离株A1和A2，在TSA中生长时能够形成生物膜，用于进一步研究。孵育3天后，在45℃、pH值7-9的条件下形成的生物膜最多。生物膜在玻璃盖玻片上生长，并与刚果红染料一起孵育，以评估它们的染料脱色能力。分离株A1表现出较高的脱色潜力，在18小时内可使80%的刚果红染料脱色，而分离株A2的效率较低，在45℃静态条件下仅能使60%的染料脱色。基于16S rRNA的分子鉴定将分离株A1鉴定为地衣芽孢杆菌A1（PV362359）。FESEM和FTIR分析等分析技术显示细菌细胞和官能团有显著变化，分别表现为峰的明显减少或消失。在1745cm、2859cm、2931cm和1154cm处的峰显示了与蛋白质和多糖相关的官能团的变化。这些表明了偶氮键和磺酸根基团的断裂。本研究表明嗜热生物膜产生细菌在偶氮染料生物修复方面的潜力。
