Muhammad Ummukulthum K, Sulyman Sulyman B, Saleh Idris, Gwarzo Muhammad Y, Babagana Kamaluddeen, Muhammad Yabagi A, Muhammad Aliyu, Ibrahim Abdulwasiu, Muhammad Suleiman A, Mohammed Aminu Z, Abdulazeez Mansurah A
Faculty of Allied Health Sciences, Department of Medical Laboratory Sciences, Bayero University, Kano, Nigeria.
Faculty of Basic Medical Sciences, Department of Biochemistry, Bayero University, Kano, Nigeria.
Breast Cancer. 2025 Aug 13. doi: 10.1007/s12282-025-01758-8.
Breast cancer constitutes 25% of all cancer cases diagnosed in Kano State, Nigeria. This study investigated the relationship between adiponectin gene polymorphisms (AGP) and adipokine and lipid profile in breast cancer patients in Kano.
The study population was composed of 80 randomly consented breast cancer patients from the three major hospitals in Kano State, Nigeria, and 40 age-matched controls across the metropolis. Biochemical assays were analyzed using standard procedures and ELISA. Furthermore, AGP was investigated using PCR-PCR-restriction fragment length polymorphism.
The incidence of breast cancer was highest in patients aged 41-50 years (25%), with a high frequency of overweight and obesity. Serum adiponectin and HDL levels were lower (p < 0.05) in the patients, whereas those of leptin, TC, TG, and LDL were higher (p < 0.05) relative to control subjects. A positive significant correlation was observed between leptin and adiponectin (r = 0.522, p < 0.0001), TG vs TC (0.446, p = 0.001), LDL vs TG (0.419, p = 0.002), and TC vs LDL (r = 0.965, p < 0.0001) in the patients. No significant association in genotype frequencies for 276G/T (χ2 = 4.35, p = 0.11) and 45 T/G (χ2 = 2.45, p = 0.12) polymorphisms between patients and controls. Furthermore, BMI was significantly associated with the 276G/T genotype.
This study suggests that the adiponectin 45 T/G and 276 G/T gene polymorphisms may not significantly influence the risk of breast cancer among patients attending these hospitals in Kano State. However, the 276 T/G genotype shows a potential association with obesity in this population. Notably, variations in adiponectin and leptin levels appear to be linked to breast cancer risk, highlighting their potential as novel biomarkers for predicting breast cancer prognosis and progression.
在尼日利亚卡诺州，乳腺癌占所有确诊癌症病例的25%。本研究调查了卡诺州乳腺癌患者脂联素基因多态性（AGP）与脂肪因子及血脂谱之间的关系。
研究人群包括来自尼日利亚卡诺州三家主要医院的80名随机同意参与研究的乳腺癌患者，以及来自该大都市的40名年龄匹配的对照者。使用标准程序和酶联免疫吸附测定法（ELISA）进行生化检测分析。此外，采用聚合酶链反应-聚合酶链反应-限制性片段长度多态性方法研究AGP。
乳腺癌发病率在41至50岁患者中最高（25%），超重和肥胖发生率较高。与对照者相比，患者血清脂联素和高密度脂蛋白（HDL）水平较低（p < 0.05），而瘦素、总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平较高（p < 0.05）。患者中，瘦素与脂联素之间（r = 0.522，p < 0.0001）、TG与TC之间（0.446，p = 0.001）、LDL与TG之间（0.419，p = 0.002）以及TC与LDL之间（r = 0.965，p < 0.0001）存在显著正相关。患者与对照者在276G/T（χ2 = 4.35，p = 0.11）和45T/G（χ2 = 2.45，p = 0.12）多态性的基因型频率上无显著关联。此外，体重指数（BMI）与276G/T基因型显著相关。
本研究表明，脂联素45T/G和276G/T基因多态性可能不会显著影响卡诺州这些医院就诊患者患乳腺癌的风险。然而，276T/G基因型在该人群中显示出与肥胖的潜在关联。值得注意的是，脂联素和瘦素水平的变化似乎与乳腺癌风险相关，突出了它们作为预测乳腺癌预后和进展的新型生物标志物的潜力。
