Zhang Xingen, Li Guohui, Dai Jun, Wei Peipei, Du Binbin, Li Fang, Wang Yulu, Wang Yujuan
Engineering Technology Research Center of Plant Cell Engineering, Lu'an 237012, China.
Plants (Basel). 2025 Jul 25;14(15):2303. doi: 10.3390/plants14152303.
Combined abiotic stresses often impose greater challenges to plant survival than individual stresses. In this study, we focused on elucidating the physiological and molecular mechanisms underlying the response of to combined salt and heat stress by integrating physiological, transcriptomic, and metabolomic analyses. Our results demonstrated that high temperature plays a dominant role in the combined stress response. Physiological assays showed increased oxidative damage under combined stress, accompanied by significant activation of antioxidant enzyme systems (SOD, POD, CAT). Metabolomic analysis revealed significant enrichment of glutathione metabolism and flavonoid biosynthesis pathways, with key antioxidants such as glutathione and naringenin chalcone accumulating under combined stress. Transcriptomic data supported these findings, showing differential regulation of stress-related genes, including those involved in reactive oxygen species scavenging and secondary metabolism. These results highlight a coordinated defense strategy in , involving both enzymatic and non-enzymatic antioxidant systems to maintain redox homeostasis under combined stress. This study provides novel insights into the molecular mechanisms underlying combined stress tolerance and lays the foundation for improving stress resilience in medicinal orchids.
与单一胁迫相比，复合非生物胁迫常常给植物的生存带来更大挑战。在本研究中，我们通过整合生理、转录组和代谢组分析，着重阐明[植物名称]对复合盐胁迫和热胁迫响应的生理及分子机制。我们的结果表明，高温在复合胁迫响应中起主导作用。生理分析显示，复合胁迫下氧化损伤增加，同时抗氧化酶系统（超氧化物歧化酶、过氧化物酶、过氧化氢酶）显著激活。代谢组分析揭示了谷胱甘肽代谢和类黄酮生物合成途径的显著富集，关键抗氧化剂如谷胱甘肽和柚皮素查耳酮在复合胁迫下积累。转录组数据支持了这些发现，显示出胁迫相关基因的差异调控，包括参与活性氧清除和次生代谢的基因。这些结果突出了[植物名称]中的一种协同防御策略，即在复合胁迫下通过酶促和非酶促抗氧化系统来维持氧化还原稳态。本研究为复合胁迫耐受性的分子机制提供了新见解，并为提高药用兰花的胁迫恢复力奠定了基础。
