生物菌剂在防治玉米上的独脚金（Striga Hermonthica (Del.) Benth）侵染中的应用。
Application of bioinoculants in combating Striga Hermonthica (Del.) Benth infestation in maize.
作者信息
Akanmu Akinlolu Olalekan, Babalola Olubukola Oluranti, Ayangbenro Ayansina Segun, Kutu Funso Raphael, Odhiambo Jude J O
机构信息
Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho, 2735, South Africa.
Imperial College London, Silwood Park Campus, Buckhurst Road, Ascot, Berkshire, SL5 7PY, UK.
出版信息
BMC Microbiol. 2025 Aug 16;25(1):512. doi: 10.1186/s12866-025-04264-1.
BACKGROUND
Striga hermonthica (Del.) Benth is an obligate root parasitic flowering plant capable of causing 30-90% yield loss in cereal fields thereby constituting a huge threat to food security. Despite its economic importance, effective and eco-friendly management measures for this weed have not yet been found. Hence, this study explores the application of bioinoculants in combating S. hermonthica infestation in maize. A total of 63 bacteria isolates originally obtained from maize rhizosphere were screened for ammonia production, siderophore production, phosphate solubilization, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and ethylene gas quantification using standard protocols. The screened bacteria isolates were subjected to both in-vitro and in-vivo experiments arranged in a completely randomized design (CRD) with three replications, to investigate the potential of the screened bioinoculant to combat against infestation in maize. Data gathered on the growth, biomass, and stalk lodging caused by Striga infestation were subjected to ANOVA analysis at p ≤ 0.05.
RESULTS
A total of 11 prospective bioinoculants were derived from the Plant-Growth Promoting Rhizobacteria (PGPR) and ethylene productions, while the in-vitro and in-vivo evaluation showed that isolates; B2 (Enterobacter asburiae OR044418), B3 (Enterobacter chengduensis, OR044419), B4 (Priestia aryabhattai OR044420), B7 (Priestia megaterium OR044423), NWUAB 12 (Pseudomonas koreensis MH399240), enhanced both growth and biomass production and exhibited strong biocontrol potential against Striga. Their performances in managing the Striga-induced damage to maize shows B3 (Enterobacter chengduensis) > B7 (Priestia megaterium) > B2 (Enterobacter asburiae) > NWUAB 12 (Pseudomonas koreensis) > B4 (Priestia aryabhattai) as the order of performance.
CONCLUSION
This research demonstrated that bioinoculants hold great promise in controlling Striga infestation.
背景
独脚金（Striga hermonthica (Del.) Benth）是一种专性根寄生开花植物，能够使谷类作物田减产30%-90%，从而对粮食安全构成巨大威胁。尽管其具有经济重要性，但尚未找到针对这种杂草的有效且环保的管理措施。因此，本研究探讨了生物菌剂在防治玉米田独脚金侵染方面的应用。使用标准方案，对最初从玉米根际获得的总共63株细菌分离株进行了氨生产、铁载体生产、磷溶解、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性和乙烯气体定量筛选。将筛选出的细菌分离株进行体外和体内实验，实验采用完全随机设计（CRD），重复三次，以研究筛选出的生物菌剂对抗玉米侵染的潜力。收集的关于独脚金侵染导致的生长、生物量和茎倒伏的数据进行p≤0.05的方差分析。
结果
总共从植物促生根际细菌（PGPR）和乙烯产生中获得了11种有前景的生物菌剂，而体外和体内评估表明，分离株B2（阿氏肠杆菌（Enterobacter asburiae）OR044418）、B3（成都肠杆菌（Enterobacter chengduensis），OR044419）、B4（阿氏Priestia aryabhattai OR044420）、B7（巨大Priestia megaterium OR044423）、西北农林科技大学AB 12（韩国假单胞菌（Pseudomonas koreensis）MH399240）促进了生长和生物量生产，并对独脚金表现出强大的生物防治潜力。它们在管理独脚金对玉米造成的损害方面的表现为B3（成都肠杆菌）>B7（巨大Priestia megaterium）>B2（阿氏肠杆菌）>西北农林科技大学AB 12（韩国假单胞菌）>B4（阿氏Priestia aryabhattai）。
结论
本研究表明生物菌剂在控制独脚金侵染方面具有巨大潜力。
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