染色体蛋白HMG1和HMG2与超螺旋DNA的差异结合。

Differential binding of chromosomal proteins HMG1 and HMG2 to superhelical DNA.

作者信息

Bustin M, Soares N

出版信息

Biochem Biophys Res Commun. 1985 Dec 17;133(2):633-40. doi: 10.1016/0006-291x(85)90952-0.

Abstract

The binding of chromosomal proteins HMG1 and HMG2 to various DNA structures was examined by a nitrocellulose filter binding assay using a 32P labelled supercoiled plasmid. Binding assays and competition experiments indicated that HMG2 has a higher affinity than HMG1 for supercoiled DNA. Studies at various ionic strengths and pH values reveal differences in the interaction of the two proteins with DNA. The results suggest that HMG1 and HMG2 are involved in distinguishable cellular functions.

摘要

通过使用32P标记的超螺旋质粒的硝酸纤维素滤膜结合试验，检测了染色体蛋白HMG1和HMG2与各种DNA结构的结合情况。结合试验和竞争实验表明，HMG2对超螺旋DNA的亲和力高于HMG1。在不同离子强度和pH值下的研究揭示了这两种蛋白质与DNA相互作用的差异。结果表明，HMG1和HMG2参与了不同的细胞功能。