Zolotova Natalia, Silina Maria, Dzhalilova Dzhuliia, Tsvetkov Ivan, Fokichev Nikolai, Makarova Olga
Department of Immunomorphology of Inflammation, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution "Petrovsky National Research Centre of Surgery", 117418 Moscow, Russia.
Department of Histology, Petrovsky Medical University, 119435 Moscow, Russia.
Toxics. 2025 Aug 21;13(8):701. doi: 10.3390/toxics13080701.
Environmental pollution with microplastics (MPs) can have a negative impact on human health. Certain findings point to the relationship between MP and the development of inflammatory bowel diseases (IBD). We investigated the effect of MP consumption on the severity of chronic colitis in male C57BL/6 mice. The MP effect was modeled by drinking water consumption with a suspension of 5 μm PS particles at a concentration of 10 mg/L replacement for 12 weeks. Chronic colitis was induced by three seven-day cycles of 1% DSS consumption (starting from the 8th, 29th and 50th days of the experiment). We investigated inflammatory infiltration, the goblet cell volume fraction and the highly sulfated and neutral mucins content in them, the endocrine cell number, the ulcerative-inflammatory process prevalence, changes in the gene's expression encoding tight junction proteins, glycocalyx components proapoptotic factor Bax and proliferation marker Mki67 in the colon, and TNFα, IL-1β, IL-6 and IL-10 cytokines content in the serum. In healthy mice, MP did not cause pathological changes in the colon; however, indirect data indicate an increase in colon permeability. In chronic colitis, MP leads to higher prevalence of all pathological changes in general, and ulcers in particular, in a greater number of crypt abscesses and enteroendocrine cells. MP consumption leads to a more severe chronic colitis course.
微塑料（MPs）造成的环境污染会对人类健康产生负面影响。某些研究结果表明MP与炎症性肠病（IBD）的发展之间存在关联。我们研究了摄入MP对雄性C57BL/6小鼠慢性结肠炎严重程度的影响。通过饮用含有浓度为10 mg/L的5μm聚苯乙烯（PS）颗粒悬浮液替代水12周来模拟MP的影响。通过三个为期七天的1%葡聚糖硫酸钠（DSS）摄入周期（从实验的第8天、第29天和第50天开始）诱导慢性结肠炎。我们研究了炎症浸润、杯状细胞体积分数及其内高度硫酸化和中性粘蛋白含量、内分泌细胞数量、溃疡性炎症过程的发生率、结肠中编码紧密连接蛋白、糖萼成分、促凋亡因子Bax和增殖标志物Mki67的基因表达变化，以及血清中TNFα、IL-1β、IL-6和IL-10细胞因子的含量。在健康小鼠中，MP不会引起结肠的病理变化；然而，间接数据表明结肠通透性增加。在慢性结肠炎中，MP总体上会导致所有病理变化的发生率更高，尤其是溃疡，隐窝脓肿和肠内分泌细胞的数量更多。摄入MP会导致慢性结肠炎病程更严重。
