脾肾阳虚型腹泻型肠易激综合征的一种机制：肠道微生物群影响ATP酶。
One mechanism of spleen-kidney yang deficiency IBS-D: intestinal microbiota affect ATPase.
作者信息
Long Qi, Li Liwen, Deng Na, Tan Zhoujin
机构信息
School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
Hunan Key Laboratory of Traditional Chinese Medicine Prescription and Syndromes Translational Medicine, Changsha, China.
出版信息
Front Microbiol. 2025 Aug 12;16:1595418. doi: 10.3389/fmicb.2025.1595418. eCollection 2025.
BACKGROUND
This study aimed to investigate the effects of adenine and combined with restraint tail-clamping stress method on the intestinal microbiota of mice with spleen-kidney yang deficiency type diarrhea predominant irritable bowel syndrome (IBS-D) from the perspective of energy metabolism.
METHODS
Twenty SPF-grade female mice were randomly divided into two groups, normal group (CN group) and model group (MD group), with 10 mice per group. A spleen-kidney yang deficiency IBS-D model was established using adenine and combined with restraint tail-clamping stress. After model establishment, enzyme-linked immunosorbent assay (ELISA) was used to detect the activity of Na-K-ATPase and Ca-Mg-ATPase; spectrophotometry was used to measure intestinal digestive enzyme activity; fluorometric diacetate (FDA) hydrolysis spectrophotometry was used to detect intestinal microbial activity; and 16S rRNA sequencing was performed to identify intestinal microbiota in the intestinal contents.
RESULTS
(1) ELISA showed a significant decrease in Na-K-ATPase and Ca-Mg-ATPase activity in spleen-kidney yang deficiency IBS-D model mice. (2) Intestinal enzyme activity analysis revealed increased lactase and amylase activity, and decreased sucrase activity in model mice. (3) Intestinal microbial activity analysis demonstrated significantly enhanced in microbial activity in spleen-kidney yang deficiency IBS-D model mice. (4) Correlation analysis revealed a significant positive correlation between and Ca-Mg-ATPase, a significant positive correlation between and Na-K-ATPase, a significant positive correlation between and sucrase, and a significant negative correlation between and sucrase.
CONCLUSION
Reduced Na-K-ATPase and Ca -Mg -ATPase activities, increased sucrase and amylase activities, decreased lactase activity, and enhanced intestinal microbial activity may constitute important mechanisms underlying the development of spleen-kidney yang deficiency IBS-D.
背景
本研究旨在从能量代谢角度探讨腺嘌呤与束缚尾夹应激法联合应用对脾肾阳虚型腹泻型肠易激综合征（IBS-D）小鼠肠道微生物群的影响。
方法
将20只SPF级雌性小鼠随机分为两组，正常组（CN组）和模型组（MD组），每组10只。采用腺嘌呤与束缚尾夹应激联合建立脾肾阳虚IBS-D模型。造模后，采用酶联免疫吸附测定（ELISA）检测钠钾ATP酶（Na-K-ATPase）和钙镁ATP酶（Ca-Mg-ATPase）活性；采用分光光度法测定肠道消化酶活性；采用荧光素二乙酸酯（FDA）水解分光光度法检测肠道微生物活性；并进行16S rRNA测序以鉴定肠内容物中的肠道微生物群。
结果
（1）ELISA显示，脾肾阳虚IBS-D模型小鼠的Na-K-ATPase和Ca-Mg-ATPase活性显著降低。（2）肠道酶活性分析显示，模型小鼠的乳糖酶和淀粉酶活性增加，蔗糖酶活性降低。（3）肠道微生物活性分析表明，脾肾阳虚IBS-D模型小鼠的微生物活性显著增强。（4）相关性分析显示，与Ca-Mg-ATPase呈显著正相关，与Na-K-ATPase呈显著正相关，与蔗糖酶呈显著正相关，与蔗糖酶呈显著负相关。
结论
Na-K-ATPase和Ca-Mg-ATPase活性降低、蔗糖酶和淀粉酶活性增加、乳糖酶活性降低以及肠道微生物活性增强可能是脾肾阳虚IBS-D发病的重要机制。
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