Miller Jonathan, Niewiesk Stefan
Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2025;2948:289-304. doi: 10.1007/978-1-0716-4666-3_19.
The hispid cotton rat (Sigmodon hispidus) has demonstrated excellent translational and predictive value in the study of respiratory syncytial virus (RSV). While the cotton rat model has many advantages over the mouse model, unfamiliarity with the species can serve as a deterrent to its use. Here, we review the most common protocols for studying RSV in the cotton rat model, including housing and handling, anesthesia and intranasal infection, drug administration, blood draws, and postmortem tissue collection. Using these basic protocols, investigators can pursue downstream applications similar to those available in other animal models, limited only by available species-specific or cross-reactive reagents.
刚毛棉鼠（Sigmodon hispidus）在呼吸道合胞病毒（RSV）研究中已展现出卓越的转化和预测价值。虽然棉鼠模型相较于小鼠模型有诸多优势，但对该物种的不熟悉可能会阻碍其应用。在此，我们回顾了在棉鼠模型中研究RSV的最常见方案，包括饲养与处理、麻醉与鼻内感染、药物给药、采血以及死后组织采集。运用这些基本方案，研究人员可以开展类似于其他动物模型中的下游应用，唯一的限制是可用的物种特异性或交叉反应试剂。
