纹石蛾（昆虫纲：毛翅目）窄体纹石蛾的染色体水平基因组组装

Chromosome-level genome assembly of the caddisfly Stenopsyche angustata (Insecta: Trichoptera).

作者信息

Wang Yujun, Liu Xinze, Qin Honglin, Zhong Xifa, Li Yimin, Qin Yuting, Wu Yueying, Zhang Yichuan, He Yuwei, Li Jisheng, Wang Hong

机构信息

Hebei Sericulture Industry Technology Innovation Center, Hebei Universities Characteristic sericulture Application Technology Research and Development Center, Sericultural Research Institute, Department of Biological Science and Technology, Chengde Medical University, Anyuan Road, Chengde, 067000, Hebei, China.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi,Ocean College, Beibu Gulf University, Qinzhou, 535000, China.

出版信息

Sci Data. 2025 Sep 1;12(1):1523. doi: 10.1038/s41597-025-05602-8.

DOI:10.1038/s41597-025-05602-8

PMID:40887518

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12399763/

Abstract

Stenopsyche angustata, a species within the diverse order Trichoptera, is widely distributed across freshwater environments and exhibits unique ecological traits that make it an ideal subject for studying adaptive evolution. In this study, we employed Illumina second-generation sequencing, PacBio third-generation sequencing, along with high-throughput chromosome conformation capture (Hi-C) technologies to generate raw sequencing data and construct chromosome-level genome assemblies of S. angustata. The final genome assembly size was 510.47 Mb, with scaffold N50 length of 39.81 Mb. The genome was successfully anchored to 13 pseudochromosomes, and a total of 10,699 protein-coding genes were identified. The genome contained 44.92% repeat sequences and 1,004 non-coding RNAs. Moreover, a total of 10,699 protein-encoding genes were predicted, representing 97.7% of the total genes. This comprehensive genomic analysis unveil crucial details about the genetic makeup of S. angustata, providing a foundation for understanding of the evolutionary processes and ecological functions of this species.

摘要

狭纹长角石蛾（Stenopsyche angustata）是种类多样的毛翅目昆虫中的一个物种，广泛分布于淡水环境中，具有独特的生态特征，使其成为研究适应性进化的理想对象。在本研究中，我们采用了Illumina二代测序、PacBio三代测序以及高通量染色体构象捕获（Hi-C）技术来生成原始测序数据，并构建狭纹长角石蛾的染色体水平基因组组装。最终的基因组组装大小为510.47 Mb，支架N50长度为39.81 Mb。该基因组成功锚定到13条假染色体上，共鉴定出10,699个蛋白质编码基因。基因组包含44.92%的重复序列和1,004个非编码RNA。此外，共预测出10,699个蛋白质编码基因，占基因总数的97.7%。这一全面的基因组分析揭示了狭纹长角石蛾基因组成的关键细节，为理解该物种的进化过程和生态功能奠定了基础。