[用放射免疫法和色谱-质谱碎片分析法对血浆中炔诺酮进行比较测定]

[Comparative determination of norethisterone in the blood plasma by radioimmunological and chromato-mass fragmentographic methods].

作者信息

Golubovskaia L E, Korenchuk E N, Pivnitskiĭ K K, Kuzovkova N A, Fanchenko N D

出版信息

Probl Endokrinol (Mosk). 1985 Nov-Dec;31(6):72-5.

Abstract

Radioimmunoassay (RIA) and two variants of the chromato-mass fragmentographic (CMF) method were used for comparative determination of norethisterone (NET) in the blood plasma. A deuterated internal standard, capillary gas-liquid chromatography and chromatographed derivates: 3-enol-3-tret-butyldimethylsilyl-17-tri-methysilyl and 3-enol-3,17-bis-tret-butyldimethylsilyl NET esters were used. Both derivates provide for CMF determination after simplified variants. They were used as references for RIA determination of NET. Comparison of the CMF and RIA results indicate to the significance of RIA determination (r = 0.979) while employing antiserum to NET conjugate 3-(0-carboxymethyl)-oxime with bovine serum albumin injected to rabbits.

摘要

采用放射免疫分析法（RIA）和两种变体的色谱 - 质谱碎片分析法（CMF）对血浆中的炔诺酮（NET）进行比较测定。使用了氘代内标、毛细管气液色谱法以及色谱衍生化产物：3 - 烯醇 - 3 - 叔丁基二甲基甲硅烷基 - 17 - 三甲基甲硅烷基和3 - 烯醇 - 3,17 - 双 - 叔丁基二甲基甲硅烷基炔诺酮酯。两种衍生化产物都可通过简化变体用于CMF测定。它们被用作RIA法测定NET的参比物。CMF和RIA结果的比较表明，在使用针对与牛血清白蛋白偶联的炔诺酮3 -（O - 羧甲基）肟注入兔子后产生的抗血清时，RIA测定具有重要意义（r = 0.979）。