低强度脉冲超声增强骨髓间充质干细胞旁分泌胰岛素样生长因子（IGF）和血管内皮生长因子（VEGF），促进成骨和血管生成。

Low-Intensity pulsed ultrasound enhances paracrine secretion of IGF and VEGF by bmscs, promoting osteogenesis and angiogenesis.

作者信息

Xu Jia, Chen Chenfeng, Gan Shuaiqi, Liao Yihan, Fu Ruijie, Hou Chuping, Yang Shuhan, Zheng Zheng, Chen Wenchuan

机构信息

State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.

Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

出版信息

BMC Musculoskelet Disord. 2025 Sep 1;26(1):828. doi: 10.1186/s12891-025-09027-7.

DOI:10.1186/s12891-025-09027-7

PMID:40890716

Abstract

BACKGROUND

Low-intensity pulsed ultrasound (LIPUS) is an effective therapy for craniofacial bone regeneration. Paracrine signaling from mesenchymal stem cells (MSCs) plays a critical role in bone repair, but the impact of LIPUS on MSC-derived secretome remains unclear. This study investigates whether LIPUS enhances the osteogenic and angiogenic potential of MSCs through modulation of growth factor secretion.

METHODS

Bone marrow-derived MSCs (BMSCs) were treated with or without LIPUS to generate conditioned media (LIPUS-CM and Ctrl-CM). Concentrations of IGF-1, VEGF, and TGF-β were quantified. These media were applied to other BMSCs and rat aortic endothelial cells (RAOECs). In vitro assays evaluated cell proliferation, migration, osteogenic differentiation, and angiogenesis. Data were analyzed using GraphPad and Image J, with significance set at α = 0.05.

RESULTS

LIPUS significantly upregulated IGF-1 and VEGF secretion in BMSCs, while TGF-β levels remained unchanged. RAOECs cultured in LIPUS-CM demonstrated enhanced proliferation, migration, and angiogenic capacity. Likewise, BMSCs cultured in LIPUS-CM showed improved proliferation, migration, and osteogenic differentiation compared to the Ctrl-CM group.

CONCLUSION

LIPUS promotes osteogenic differentiation of BMSCs and angiogenesis in RAOECs through the paracrine signaling of BMSCs, at least by increasing IGF-1 and VEGF secretion. This suggests that LIPUS can regulate the secretome of BMSCs and may serve as a key mechanism in promoting bone tissue regeneration.

摘要

背景

低强度脉冲超声（LIPUS）是颅面骨再生的一种有效治疗方法。间充质干细胞（MSC）的旁分泌信号在骨修复中起关键作用，但LIPUS对MSC衍生的分泌组的影响尚不清楚。本研究调查LIPUS是否通过调节生长因子分泌来增强MSC的成骨和血管生成潜力。

方法

用或不用LIPUS处理骨髓来源的MSC（BMSC）以产生条件培养基（LIPUS-CM和Ctrl-CM）。定量IGF-1、VEGF和TGF-β的浓度。将这些培养基应用于其他BMSC和大鼠主动脉内皮细胞（RAOEC）。体外试验评估细胞增殖、迁移、成骨分化和血管生成。使用GraphPad和Image J分析数据，显著性设定为α = 0.05。