Glutathione S-transferase in the differentiation of adventitious buds from callus.

INTRODUCTION

holds cultural and horticultural significance in traditional Chinese gardens, as a regional endemic species in Yunnan Province. However, during the regeneration process of , there is often a phenomenon of low efficiency of adventitious bud differentiation or no adventitious bud differentiation at all.

METHODS

In previous study, we observed significant morphological differences between the callus tissues of 'Zipao' and wild species. The callus of 'Zipao' was white and loosely textured, the wild species was green and hard. To investigate the differences between these two types of callus, we conducted transcriptome analysis and identified a differentially expressed gene , which is closely related to the synthesis of glutathione (GSH). Heterologous transformation of into tobacco () was conducted.

RESULTS AND DISCUSSION

The gene promoted the synthesis of endogenous auxin and cytokinin in transgenic tobacco by regulating the expression of transcription factors related to auxin and cytokinin. Exogenous IBA, 6-BA, and red-blue light treatment increased the levels of auxins and cytokinins in callus, thereby promoting adventitious bud differentiation. Additionally, interacted with and genes, facilitating GSH and AsA synthesis and clearing ROS.