Chi Yan-Yu, Xiang Jun-Yan, Li Hui-Min, Shi Hao-Yu, Ning Ke, Xiang Hongyu, Xie Qiuhong
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, China.
Leeds Institute of Data Analytics, University of Leeds, Leeds, UK.
Curr Res Food Sci. 2025 Aug 19;11:101172. doi: 10.1016/j.crfs.2025.101172. eCollection 2025.
Alcohol-associated liver disease (ALD) has emerged as a global public health concern, and gut microbiota plays a decisive role in its pathogenesis. , a functional edible berry, exhibits hepatoprotective properties. However, the preventive effects of its main active component lignans against ALD, as well as their connections with gut microbiota and intestinal metabolites remain unclear. This study employed a mouse model of ALD induced by chronic-binge ethanol feeding to evaluate the hepatoprotective effects of lignans-rich extract of (SCL) interventions at different doses. 16S rDNA sequencing and metabolomic analysis were used to investigate microbial and metabolite profiles in intestinal contents. The hepatoprotective activity of key metabolite responding to SCL was further verified by and experiments. The results demonstrated that SCL effectively restored intestinal barrier function and alleviated alcohol-induced hepatic injury. SCL modulated intestinal tryptophan (Trp) metabolism and restored indole-3-acetic acid (IAA) levels. Concurrently, SCL reshaped the gut microbiota composition, enriched . . Further and investigations demonstrated that SCL-mediated enrichment of contributed to intestinal IAA restoration which reactivated the ethanol-suppressed AHR pathway, enhanced intestinal barrier integrity, reduced lipopolysaccharide translocation into circulation and ultimately attenuates liver injury. Collectively, SCL prevent ALD by regulating intestinal microbiota and Trp metabolism. This study provides a scientific foundation for the development of as a functional food for ALD prevention.
酒精性肝病（ALD）已成为全球公共卫生关注的问题，肠道微生物群在其发病机制中起决定性作用。[某种功能性可食用浆果]具有肝脏保护特性。然而，其主要活性成分木脂素对ALD的预防作用，以及它们与肠道微生物群和肠道代谢物的联系仍不清楚。本研究采用慢性暴饮乙醇喂养诱导的ALD小鼠模型，评估不同剂量的[该浆果富含木脂素的提取物（SCL）]干预的肝脏保护作用。利用16S rDNA测序和代谢组学分析来研究肠道内容物中的微生物和代谢物谱。通过[具体实验1]和[具体实验2]实验进一步验证了对SCL有反应的关键代谢物的肝脏保护活性。结果表明，SCL有效地恢复了肠道屏障功能，减轻了酒精诱导的肝损伤。SCL调节肠道色氨酸（Trp）代谢并恢复吲哚-3-乙酸（IAA）水平。同时，SCL重塑了肠道微生物群组成，富集了[具体微生物种类]。进一步的[具体实验3]和[具体实验4]研究表明，SCL介导的[具体微生物种类]富集有助于肠道IAA恢复，从而重新激活乙醇抑制的芳烃受体（AHR）途径，增强肠道屏障完整性，减少脂多糖向循环中的转运，并最终减轻肝损伤。总体而言，SCL通过调节肠道微生物群和Trp代谢来预防ALD。本研究为将[该浆果]开发为预防ALD的功能性食品提供了科学依据。
