Qin Yao, Yuan Shichao, Wang Xihan, Zhang Qinyi, Wu Jie, Zhang Hongbin, Li Ling
Guangdong Medical University, Dongguan, 523520, China.
General Hospital of Southern Theater Command of PLA, Guangzhou, 510010, China.
BMC Infect Dis. 2025 Sep 3;25(1):1097. doi: 10.1186/s12879-025-11490-5.
A novel paper-based electrochemiluminescence test strip (ECL-TS) for the detection of pathogenic microorganisms is developed by combining lateral flow immunochromatography(LFIC) with ECL.
Based on the principle of double-antibody sandwich, monoclonal antibody 1 labeled with tris(bipyridine)ruthenium is fixed on the conjugate pad as the labeled antibody, and monoclonal antibody 2 is directly fixed on the detection pad as the capture antibody. The antibody is Made to flow to the detection region through LFIC and specifically bind to the capture antibody in the detection region. Cyclic voltammetry scanning is carried out at a speed of 0.1 V/s in the detection region to obtain an ECL signal, which is then converted into the content of the virus in the sample to be tested.
Under the optimized conditions, the developed test strip could be detected within 5 min, with a minimum detection Limit of 7.96 pg/mL, a reproducibility deviation of 5.62%, a stability deviation of 6.34%, and a KAPPA value of 0.88, which was specific for the related viruses.
The developed paper-based ECL-TS provides a portable, rapid and sensitive detection method. Taking the novel coronavirus (SARS-CoV-2) as an example, compared with the nucleic acid detection method, this method has the advantages of being rapid, accurate and low-cost. It is expected to be used as a point-of-care testing (POCT) tool in the detection of pathogenic microorganisms.
通过将侧向流免疫层析法（LFIC）与电化学发光（ECL）相结合，开发了一种用于检测病原微生物的新型纸质电化学发光测试条（ECL-TS）。
基于双抗体夹心原理，将用三联吡啶钌标记的单克隆抗体1作为标记抗体固定在结合垫上，单克隆抗体2作为捕获抗体直接固定在检测垫上。使抗体通过LFIC流向检测区域，并与检测区域中的捕获抗体特异性结合。在检测区域以0.1 V/s的速度进行循环伏安扫描以获得ECL信号，然后将其转换为待测样品中病毒的含量。
在优化条件下，所开发的测试条可在5分钟内检测到，最低检测限为7.96 pg/mL，重现性偏差为5.62%，稳定性偏差为6.34%，KAPPA值为0.88，对相关病毒具有特异性。
所开发的纸质ECL-TS提供了一种便携式、快速且灵敏的检测方法。以新型冠状病毒（SARS-CoV-2）为例，与核酸检测方法相比，该方法具有快速、准确和低成本的优点。有望用作病原微生物检测中的即时检测（POCT）工具。
