Hassall D G, Bruckdorfer K R, Sulpice J C, Lutton C
Thromb Haemost. 1985 Dec 17;54(4):760-3.
It has been reported that lanosterol can sensitize isolated rat platelets to agonists such as ADP and thrombin (4). The purpose of this paper was to determine whether lanosterol had similar effects on human platelets and whether this was achieved by changes in membrane fluidity. Lanosterol did increase the sensitivity of human platelets, particularly to adrenaline and ADP at concentrations as low as 5 mg.L-1 when added from solutions in ethanol. At similar concentrations cholesterol, 4-cholesten-3-one or ethynyloestradiol had either no effect or were inhibitory. Measurement of membrane fluidity with diphenylhexatriene indicated that lanosterol did not affect membrane fluidity. Incubation of platelets with [4C]-mevalonic acid gave rise to a very small incorporation into lanosterol, squalene and farnesol. Sudden activation of the platelets did not accelerate lanosterol synthesis during or after platelet aggregation. It was concluded that lanosterol could only influence platelet behaviour if it came from the plasma. However the concentration of the steroid in both platelets and plasma is ten fold less than that required to sensitise the platelets.
据报道，羊毛甾醇可使分离的大鼠血小板对二磷酸腺苷（ADP）和凝血酶等激动剂敏感（4）。本文的目的是确定羊毛甾醇对人血小板是否有类似作用，以及这是否通过膜流动性的改变来实现。羊毛甾醇确实增加了人血小板的敏感性，尤其是当从乙醇溶液中添加低至5mg.L-1浓度的肾上腺素和ADP时。在相似浓度下，胆固醇、4-胆甾烯-3-酮或乙炔雌二醇要么没有作用，要么具有抑制作用。用二苯基己三烯测量膜流动性表明，羊毛甾醇不影响膜流动性。用[4C]-甲羟戊酸孵育人血小板后，仅有极少量掺入羊毛甾醇、角鲨烯和法尼醇。血小板的突然激活在血小板聚集期间或之后并未加速羊毛甾醇的合成。得出的结论是，羊毛甾醇只有来自血浆时才能影响血小板行为。然而，血小板和血浆中该类固醇的浓度比使血小板敏感所需的浓度低十倍。
