倍半萜内酯 hymenoxon 对脱氧鸟苷的烷基化作用。

Alkylation of deoxyguanosine by the sesquiterpene lactone hymenoxon.

作者信息

Sylvia V L, Joe C O, Stipanovic R D, Kim H L, Busbee D L

出版信息

Toxicol Lett. 1985 Dec;29(2-3):69-76. doi: 10.1016/0378-4274(85)90026-8.

Abstract

Hymenoxon, a toxic sesquiterpene lactone found in the ruminant forage plant Hymenoxys odorata, binds deoxyguanosine in a cell-free system, and forms adducted guanine residues in sheep lymphocyte DNA. Mitogen-stimulated DNA synthesis in lymphocytes was inhibited by hymenoxon at concentrations greater than 100 microM. Unscheduled DNA synthesis in lymphocytes was initiated by hymenoxon concentrations exceeding 50 microM, and inhibited by concentrations above 100 microM. We describe an HPLC method which separates unmodified hymenoxon and deoxyguanosine from the hymenoxon-deoxyguanosine adduct, and allows the quantitation of adducts in hymenoxon-treated cells.

摘要

雷公藤红素是一种在反刍动物饲料植物毛叶金光菊中发现的有毒倍半萜内酯，在无细胞体系中，它可与脱氧鸟苷结合，并在绵羊淋巴细胞DNA中形成加合的鸟嘌呤残基。当浓度高于100微摩尔时，雷公藤红素可抑制淋巴细胞中由丝裂原刺激的DNA合成。当浓度超过50微摩尔时，雷公藤红素可引发淋巴细胞的DNA非常规合成，而当浓度高于100微摩尔时则产生抑制作用。我们描述了一种高效液相色谱法，该方法可将未修饰的雷公藤红素和脱氧鸟苷与雷公藤红素-脱氧鸟苷加合物分离，并能对经雷公藤红素处理的细胞中的加合物进行定量分析。