基于细胞色素B基因的印度北部环形泰勒虫小牛分离株的联合遗传多样性
Associative Genetic Diversity of Theileria Annulata Calf Isolates from Northern India Based on Cyt B Gene.
作者信息
Kumar Sanjeev, Shanker Daya, Sudan Vikrant, Singh Shanker Kumar
机构信息
Department of Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
Department of Veterinary Parasitology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Rampura Phul, Punjab, 151103, India.
出版信息
Acta Parasitol. 2025 Sep 3;70(5):190. doi: 10.1007/s11686-025-01126-8.
PURPOSE
Molecular epidemiological studies focusing on the phylogenetic characterization of Theileria annulata are crucial for understanding the evolutionary history of the parasite worldwide. The current study reveals genetic diversity among Indian isolates of T. annulata based on the cyt b gene.
METHOD
In the present study, cyt b gene from 6 calf isolates of T. annulata was amplified; custom sequenced and accession numbers: MH778940-MH778945 were obtained.
RESULT
Two haplotypes were identified, differing by nucleotide substitutions at positions 710 (thymine to adenine) and 1076 (cytosine to guanine). Isolates from Northern India formed a distinct cluster on the phylogenetic tree compared to those from Southern India and showed closer phylogenetic similarity to Iranian isolates than to other Asian counterparts.
CONCLUSION
Important phylogenetic data has been generated from the present study suggestive of marked genetic variability in T. annulata isolates across the globe.
目的
聚焦环形泰勒虫系统发育特征的分子流行病学研究对于理解该寄生虫在全球的进化史至关重要。当前研究基于细胞色素b基因揭示了印度环形泰勒虫分离株之间的遗传多样性。
方法
在本研究中，对6株环形泰勒虫小牛分离株的细胞色素b基因进行了扩增；进行了定制测序并获得了登录号：MH778940 - MH778945。
结果
鉴定出两种单倍型，在第710位（胸腺嘧啶替换为腺嘌呤）和第1076位（胞嘧啶替换为鸟嘌呤）存在核苷酸替换差异。与印度南部的分离株相比，印度北部的分离株在系统发育树上形成了一个独特的聚类，并且与伊朗分离株的系统发育相似性比与其他亚洲分离株更近。
结论
本研究产生了重要的系统发育数据，表明全球环形泰勒虫分离株存在显著的遗传变异性。
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