Zhao Yan, Xue Ge, Peng Yanghui, Zhang Jia, Chen Feng, Wang Yeke, He Jun, Chen Jun, Xie Ping
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Biology (Basel). 2025 Aug 3;14(8):987. doi: 10.3390/biology14080987.
Spawning diversity plays an essential role in fish survival and reproduction, which contributes to the exceptional diversity of teleosts among vertebrates. Different zona radiata structures reflect the adaptability of fish to the environment of spawning and early embryonic development. The morphological and transcriptional characteristics of fish follicle development between different spawning habits, particularly the zona radiata variations, have been poorly documented. In this study, we integrated histology and transcriptomics to investigate the differences in the zona radiata structure and gene expression profiles among follicles from different spawning habits of . Our results revealed that stage Ⅲ was the crucial period for zona radiata thickening and structure differentiation. Transcriptomic analyses of adhesive and semi-buoyant eggs at stage Ⅲ revealed a significant upregulation of genes involved in glycoprotein synthesis, extracellular matrix formation, and regulation of protease activity in adhesive eggs, such as the and gene family. This upregulation likely underpins the thicker zona radiata in adhesive eggs, facilitating their attachment to substrates. This study represents the first elucidation of the ultrastructure of the zona radiata and gene expression patterns in different developmental stages of adhesive and semi-buoyant eggs of , offering new perspectives for aquaculture research in understanding fish reproductive adaptations.
产卵多样性在鱼类生存和繁殖中起着至关重要的作用，这促成了硬骨鱼在脊椎动物中具有非凡的多样性。不同的放射带结构反映了鱼类对产卵环境和早期胚胎发育的适应性。不同产卵习性之间鱼类卵泡发育的形态学和转录特征，尤其是放射带的变化，鲜有文献记载。在本研究中，我们整合了组织学和转录组学，以研究不同产卵习性的卵泡之间放射带结构和基因表达谱的差异。我们的结果表明，Ⅲ期是放射带增厚和结构分化的关键时期。对Ⅲ期粘性卵和半浮性卵的转录组分析显示，粘性卵中参与糖蛋白合成、细胞外基质形成和蛋白酶活性调节的基因显著上调，如 和 基因家族。这种上调可能是粘性卵放射带更厚的原因，有助于它们附着在基质上。本研究首次阐明了 粘性卵和半浮性卵不同发育阶段放射带的超微结构和基因表达模式，为水产养殖研究理解鱼类生殖适应性提供了新的视角。
