Changes in galectin-1 expression in pulp tissue and CD68 + macrophages in irreversible pulpitis and healthy pulp.
作者信息
Mestas-Ramos José Antonio, Franco-Arellanes María Cristina, Ambrosio-Castillo Luis Enrique, González-González Ulises, Hernández-Aguilar María Elena, Ramírez-Thomé Saira Karina, Hernández-Juárez Jesús, Ávila-Curiel Beatriz Xóchitl, Zenteno Edgar, Solórzano-Mata Carlos Josué
机构信息
Faculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Mexico.
Faculty of Medicine and Surgery, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Mexico.
出版信息
J Mol Histol. 2025 Sep 5;56(5):294. doi: 10.1007/s10735-025-10583-4.
UNLABELLED
Galectin-1 is a protein from the lectin family that is capable of recognizing β-galactosides, and it is involved in modulating the inflammatory response and tissue homeostasis. However, the presence and distribution of galectin-1 in pulp tissue, as well as its role in pulp inflammation, are poorly understood. Although galectin-1 has been reported to be present in healthy and necrotic pulp tissue at the proteomic level, the modifications and implications of these changes in galectin in tissues with irreversible pulpitis and infiltrating macrophages that could help clarify the inflammatory phenomenon have not yet been described.
OBJECTIVE
To determine the presence and distribution of galectin-1 in the dental pulp and in macrophages in tissues with irreversible pulpitis and healthy pulp.
MATERIALS AND METHODS
Immunofluorescence assays were performed on tissues from patients with irreversible pulpitis and their healthy counterparts to explore the presence of galectin-1 in different pulp regions. In addition, galectin-1 was detected in tissue macrophages (CD68 +). Images were analyzed via Fiji/ImageJ software.
RESULTS
Our data revealed that galectin-1 is expressed in both healthy pulp tissue and tissue with irreversible pulpitis, with a greater intensity of galectin-1 in tissues with irreversible pulpitis. Odontoblasts and macrophages (CD68 +) colocalized with galectin-1. Our findings indicate a higher number of CD68 + macrophages in the peripheral region of healthy pulp tissue, and that Gal-1 is expressed in these cells under both inflammatory and non-inflammatory conditions.
CONCLUSION
Galectin-1 is present in tissues from patients with irreversible pulpitis and their healthy counterparts. Gal-1 is a molecule anti-inflammatory, suppressive, and pro-resolving functions, Gal-1 could potentially mitigate the pulpal inflammatory response and influence macrophage M2 polarization with the aim of preserving pulp vitality. Elucidating the precise function of galectin-1 in dental pulp will be a crucial focus of future research.
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