Bani-Ahmad Mohammad A, Al-Husein Belal A, Alshaabi Diala Q, Aldmour Duaa A, Ghanim Yasmeen E, Al Deqah Rahaf F
Department of Medical Laboratory Science, Faculty of Applied Medical Science, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Brain Behav Immun Health. 2025 Aug 28;48:101095. doi: 10.1016/j.bbih.2025.101095. eCollection 2025 Oct.
Polysubstance use disorder (PSUD) is a chronic disease with adverse clinical outcomes. Hematological properties and related inflammatory biomarkers have not been investigated in PSUD. This study aimed to investigate the alterations in hematologic and related inflammatory parameters among subjects with PSUD.
A total of sixty subjects were included, thirty chronic PSUD subjects and thirty control subjects. Venous blood was withdrawn from participants, and complete blood count was conducted. Related inflammatory biomarkers were calculated, including neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), neutrophil-to-monocyte ratio (NMR), derived neutrophil-to-lymphocyte ratio (dNLR), and systemic inflammation index (SII).
Between study groups, we reported significant differences in red cell count, hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration (P < 0.001). As compared to control subjects, PSUD subjects had significantly higher neutrophil counts that coincided with significantly lower monocyte counts (P < 0.001). These findings corresponded to significantly higher NLR (2.88 ± 0.21 vs. 1.92 ± 0.13), dNLR (2.39 ± 0.97 vs.1.43 ± 0.08), NMR (35.1 ± 4.8 vs.8.3 ± 0.5), LMR (13.3 ± 1.7 vs. 4.6 ± 0.3) and SII (683.0 ± 56.9 vs. 424.3 ± 30.2) among PSUD subjects (P < 0.001). Receiver operating characteristic analysis revealed areas under the curves for NMR, LMR, dNLR, NLR, and SII of 0.983, 0.829, 0.811, 0.766, and 0.779, respectively (P < 0.001).
Chronic PSUD alters erythrocyte indices that define mild erythrocytic hyperchromasia and may suggest membrane damage. Furthermore, higher hematological inflammatory biomarkers imply the contribution of systemic inflammation in the pathophysiology of PSUD and suggest their diagnostic predictivity of the disease.
多物质使用障碍（PSUD）是一种具有不良临床结局的慢性疾病。尚未对PSUD患者的血液学特性及相关炎症生物标志物进行研究。本研究旨在调查PSUD患者血液学及相关炎症参数的变化。
共纳入60名受试者，其中30名慢性PSUD患者和30名对照受试者。采集参与者的静脉血并进行全血细胞计数。计算相关炎症生物标志物，包括中性粒细胞与淋巴细胞比值（NLR）、淋巴细胞与单核细胞比值（LMR）、中性粒细胞与单核细胞比值（NMR）、衍生中性粒细胞与淋巴细胞比值（dNLR）和全身炎症指数（SII）。
在研究组之间，我们发现红细胞计数、血细胞比容、平均红细胞血红蛋白含量和平均红细胞血红蛋白浓度存在显著差异（P < 0.001）。与对照受试者相比，PSUD患者的中性粒细胞计数显著更高，同时单核细胞计数显著更低（P < 0.001）。这些结果对应于PSUD患者中显著更高的NLR（2.88 ± 0.21对1.92 ± 0.13）、dNLR（2.39 ± 0.97对1.43 ± 0.08）、NMR（35.1 ± 4.8对8.3 ± 0.5）、LMR（13.3 ± 1.7对4.6 ± 0.3）和SII（683.0 ± 56.9对424.3 ± 30.2）（P < 0.001）。受试者工作特征分析显示，NMR、LMR、dNLR、NLR和SII的曲线下面积分别为0.983、0.829、0.811、0.766和0.779（P < 0.001）。
慢性PSUD会改变定义轻度红细胞高色素血症的红细胞指数，并可能提示膜损伤。此外，较高的血液学炎症生物标志物意味着全身炎症在PSUD病理生理学中的作用，并提示它们对该疾病的诊断预测性。
