辐射诱导溶解于有机溶剂中的脂质发生过氧化反应及其受α-生育酚和千金藤素的抑制作用。

Radiation-induced peroxidation of lipid dissolved in organic solvent and its inhibition by alpha-tocopherol and cepharanthine.

作者信息

Shiraishi N, Joja I, Kuroda M, Fujishima M, Miyake M, Aono K

出版信息

Physiol Chem Phys Med NMR. 1985;17(3):243-6.

Abstract

Effects of cepharanthine and alpha-tocopherol on radiation-induced peroxidation of lipids dissolved in methanol(MeOH)-chloroform (CHCl3)-H2O(v/v, 2/1/0.8) were examined. alpha-Tocopherol strongly inhibited radiation-induced peroxidation of lipids dissolved in MeOH-CHCl3-H2O. However, cepharanthine exhibited a weak inhibitory action in this system. The change in the absorption spectrum of alpha-tocopherol and cepharanthine by X-irradiation was measured. The reagents were dissolved in 95% EtOH acidified with 20 mM HCl and in MeOH-CHCl3-H2O. alpha-Tocopherol exhibited the change in its absorption spectrum in both systems, and seemed to be oxidized at a high rate by free radicals. However, cepharanthine slightly exhibited the change in its spectrum in MeOH-CHCl3-H2O, but not in acidified EtOH.

摘要

研究了千金藤素和α-生育酚对溶解于甲醇（MeOH）-氯仿（CHCl3）-水（v/v，2/1/0.8）中的脂质辐射诱导过氧化作用的影响。α-生育酚强烈抑制溶解于MeOH-CHCl3-水的脂质的辐射诱导过氧化。然而，千金藤素在该体系中表现出较弱的抑制作用。测量了X射线照射下α-生育酚和千金藤素吸收光谱的变化。试剂分别溶解于用20 mM HCl酸化的95%乙醇以及MeOH-CHCl3-水中。α-生育酚在两个体系中均表现出吸收光谱的变化，似乎被自由基以高速率氧化。然而，千金藤素在MeOH-CHCl3-水中吸收光谱略有变化，但在酸化乙醇中则无变化。