Ferdous Jannatul, Hossain Md Saddam, Sarker Palash Kumar, Bhuia Md Shimul, Al Hasan Md Sakib, Chowdhury Raihan, Ali Md Sarafat, Auwal Md Robiul, Das Keshob Chandra, Islam Muhammad Torequl, Hashem Abu, Salimullah Md
Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Department of Biotechnology and Genetic Engineering, Gopalganj Science and Technology University, Gopalganj, 8100, Bangladesh.
Probiotics Antimicrob Proteins. 2025 Sep 24. doi: 10.1007/s12602-025-10775-0.
Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.
益生菌是有益的活微生物，可能为多种疾病（如腹泻）提供安全的治疗方法。当前的研究旨在通过体外和体内方法评估潜在益生菌的止泻活性。从乳制品中分离出细菌菌株并进行益生菌特性筛选，基于体外方法选择了四株菌株，即困惑魏斯氏菌（ProY 5.1）、乳酸肠球菌（ProY 5.3）、乳酸明串珠菌（RM 2.1）和枯草芽孢杆菌（ProY 11.3）。这些菌株表现出非溶血特性、耐酸性、对胆盐和苯酚的耐受性、强黏附率、益生菌活性以及对六种肠道病原体的抗菌活性。通过应用分子技术（基质辅助激光解吸电离飞行时间质谱和全基因组测序）鉴定潜在菌株。在蓖麻油诱导腹泻的瑞士白化小鼠上进行的体内实验表明，与作为阳性对照的洛哌丁胺和次水杨酸铋相比有积极影响，而PBS + MRS肉汤用作阴性对照。筛选出的ProY 5.1、ProY 5.3和RM 2.1显著延长了潜伏期并减轻了腹泻症状（p < 0.005）。在ProY 11.3分离株中发现相对较低的止泻活性。候选益生菌的组合比单独使用表现出更强的活性。总之，这三株菌株可能是治疗腹泻的潜在益生菌候选菌株。
