IgG B细胞上CD23表达强度反映哮喘患者血清IgE水平。
Intensity of CD23 expression on IgG B cells reflects serum IgE level in asthma.
作者信息
Baert Laurie, Wiest Matthew, Yang Agnes, Upchurch Katherine, Millard Mark, Joo HyeMee, Oh SangKon
机构信息
Department of Immunology, Mayo Clinic, Scottsdale, Ariz.
Institute for Biomedical Studies, Baylor University, Waco, Tex.
出版信息
J Allergy Clin Immunol Glob. 2025 Aug 28;4(4):100562. doi: 10.1016/j.jacig.2025.100562. eCollection 2025 Nov.
BACKGROUND
IgE plays a key role in asthma pathogenesis. To produce high-affinity IgE, B cells can undergo IgE class switching through an IgG intermediate stage with repeated antigenic stimulation. Nonetheless, the phenotype of B cells that holds IgE response in asthma patients remains to be fully investigated.
OBJECTIVES
We investigated whether IgG B cells reflect the magnitude of the IgE response and correlate with asthma phenotype and disease severities in adult asthma patients.
METHODS
The frequencies of IgG , CD23, and CD23IgG B cells, along with surface CD23 expression levels in the blood of adult asthma patients (n = 40), were compared with those of nonasthmatic control subjects (n = 24). We then investigated whether the frequencies of individual B-cell populations and their surface CD23 expression levels were linked to serum IgE concentrations, eosinophil counts, and lung functions. Additionally, we also investigated whether serum IgE could affect the frequencies of these B-cell populations in patients with moderate-to-severe asthma (n = 16).
RESULTS
Serum IgE concentrations correlated with CD23 expression levels on CD23IgG B cells, but not their numbers that were linked to blood eosinophil counts and lung functions (forced expiratory volume in 1 second). Neither the frequency of CD23IgG B cells nor the CD23 expression levels were affected by steroid treatment or leukotriene inhibitors. Neutralizing IgE with omalizumab did not alter the frequency of CD23, IgG , or CD23IgG B cells in asthma patients.
CONCLUSIONS
Surface CD23 expression levels on CD23IgG B cells, but not their numbers, correlate with the magnitude of IgE response in adult asthma patients.
背景
IgE在哮喘发病机制中起关键作用。为产生高亲和力IgE，B细胞可通过IgG中间阶段经反复抗原刺激进行IgE类别转换。然而，哮喘患者中维持IgE应答的B细胞表型仍有待充分研究。
目的
我们研究了IgG⁺ B细胞是否反映成年哮喘患者IgE应答的强度，并与哮喘表型及疾病严重程度相关。
方法
将40例成年哮喘患者血液中IgG⁺、CD23⁺及CD23⁺IgG⁺ B细胞的频率以及表面CD23表达水平与24例非哮喘对照受试者进行比较。然后我们研究了各个B细胞群体的频率及其表面CD23表达水平是否与血清IgE浓度、嗜酸性粒细胞计数及肺功能相关。此外，我们还研究了血清IgE是否会影响中重度哮喘患者（n = 16）中这些B细胞群体的频率。
结果
血清IgE浓度与CD23⁺IgG⁺ B细胞上的CD23表达水平相关，但与嗜酸性粒细胞计数及肺功能（1秒用力呼气量）相关的其数量无关。CD23⁺IgG⁺ B细胞的频率及CD23表达水平均不受类固醇治疗或白三烯抑制剂影响。使用奥马珠单抗中和IgE并未改变哮喘患者中CD23⁺、IgG⁺或CD23⁺IgG⁺ B细胞的频率。
结论
成年哮喘患者中，CD23⁺IgG⁺ B细胞的表面CD23表达水平而非其数量与IgE应答强度相关。
Zotero 插件