Sander G, Jacobs J P
J Clin Pathol. 1972 May;25(5):441-2. doi: 10.1136/jcp.25.5.441.
During the course of some routine immunofluorescence studies ;non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (;passages') in species-homologous cells subjected to serial cultivation.
在一些常规免疫荧光研究过程中;在没有游离荧光素和任何已知杂质的情况下出现了“非特异性”染色。这种染色的原因似乎与在连续培养的同种细胞中不同群体倍增(“传代”)时发生的阶段性变化有关。