Immunoelectrophoretic and immunodiffusion studies on the seminal plasma proteins in boars.

The proteins of boar seminal plasma have been studied with immunoelectrophoresis and immunodiffusion tests. Four different proteins could be identified with immunoelectrophoresis of which all seemed to be specific for seminal plasma. One of these proteins with an electrophoretic mobility corresponding to gammaglobulin had no identity with either IgG or fibrinogen. With immunodiffusion tests spur of serum proteins could be detected (albumin, transferrin and IgG). The spontaneous precipitation of boar seminal plasma in agar gels could not wholly be avoided with alterations of pH and ionic strength in the agar gel or with absorption of seminal plasma with swine red blood cells. One factor in seminal plasma which probably is of importance for the ability to agglutinate red blood cells was isolated and identified as a protein with an electrophoretic mobility corresponding to alphaglobulin. This protein could not be detected in blood serum.