Horne C H, Thompson W D, Busuttil A, MacSween R N
Br J Exp Pathol. 1973 Apr;54(2):222-8.
A radial immunodiffusion technique has been used to measure the levels of 4 serum proteins, albumin, transferrin, slow α-globulin and γ-globulin in rats before, during and after the administration of carbon tetrachloride and sodium phenobarbitone for 9 weeks and in control rats receiving carbon tetrachloride alone, phenobarbitone alone, or no treatment. In the rats receiving both sodium phenobarbitone and carbon tetrachloride or carbon tetrachloride alone, there were continuous significant falls in serum albumin and transferrin levels during the 15 weeks following cessation of treatment. In contrast, both these groups of rats showed significant increases in slow α-globulin and γ-globulin levels after cessation of treatment. There were significant increases in albumin, transferrin and γ-globulin levels in the sodium phenobarbitone treated group. In the untreated group a significant increase in γ-globulin was observed. The striking serum protein changes observed in the rats following administration of sodium phenobarbitone and carbon tetrachloride alone or together are likely to be the result of persisting functional liver cell damage, whether accompanied by cirrhosis or not.
采用放射免疫扩散技术，对大鼠在给予四氯化碳和苯巴比妥钠9周期间及之后，以及单独给予四氯化碳、单独给予苯巴比妥钠或未接受任何处理的对照大鼠，检测血清白蛋白、转铁蛋白、慢α球蛋白和γ球蛋白这4种血清蛋白的水平。在同时接受苯巴比妥钠和四氯化碳的大鼠以及单独接受四氯化碳的大鼠中，停止治疗后的15周内血清白蛋白和转铁蛋白水平持续显著下降。相比之下，这两组大鼠在停止治疗后慢α球蛋白和γ球蛋白水平均显著升高。在苯巴比妥钠治疗组中，白蛋白、转铁蛋白和γ球蛋白水平显著升高。在未治疗组中，观察到γ球蛋白显著增加。在单独或同时给予苯巴比妥钠和四氯化碳的大鼠中观察到的显著血清蛋白变化，可能是持续存在的功能性肝细胞损伤的结果，无论是否伴有肝硬化。
