Laskov R, Scharff M D
J Exp Med. 1974 Oct 1;140(4):1112-6. doi: 10.1084/jem.140.4.1112.
The rates of immunoglobulin synthesis have been examined in two MFC-11 cell lines which were independently adapted to tissue culture and in light-chain-producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.
在两个独立适应组织培养的MFC - 11细胞系及其各自衍生的轻链产生变体中,检测了免疫球蛋白的合成速率。一个细胞系每细胞每小时合成2.5皮克免疫球蛋白,而另一个细胞系每细胞每小时合成3.6皮克免疫球蛋白。两个细胞系中重链和轻链的比例大致相同,免疫球蛋白细胞内池的大小与合成速率成比例。自发丧失产生重链能力的变体继续以与其亲本细胞系大致相同的速率合成轻链。