Lemeshko V V, Kudokotseva E V, Belous A M
Biokhimiia. 1978 Jan;43(1):72-7.
The effects of freezing of microsomes in liquid nitrogen and those of storage of microsomal suspensions at 2-4 degrees C and -3 - -5 degrees C for 24 hrs, on the enzymatic activities and hydrophobicity of membranes were studied. The hydrophobicity was determined by fluorescence of bound 1,8-anilino-naphthalene sulfonate. Rapid freezing of the microsomal suspension in liquid nitrogen followed by rapid warming did not change the hydrophobicity of the membranes, the rate of enzymatic lipid peroxidation, the level of cytochrome P-450 and the activity of NADH- and NADPH-cytochrome c reductase. A considerable decrease in the rate of enzymatic lipid peroxidation and membrane hydrophobicity was observed in the microsomes stored for 24 hrs at 2-4 degrees C. The 24-hr storage at -3 - -5 degrees C with subsequent thawing resulted in a rapid aggregation of the microsomes.
研究了微粒体在液氮中冷冻以及微粒体悬浮液在2-4℃和-3--5℃下储存24小时对膜的酶活性和疏水性的影响。通过结合的1,8-苯胺基萘磺酸盐的荧光测定疏水性。微粒体悬浮液在液氮中快速冷冻后快速升温，并未改变膜的疏水性、酶促脂质过氧化速率、细胞色素P-450水平以及NADH-和NADPH-细胞色素c还原酶的活性。在2-4℃下储存24小时的微粒体中，酶促脂质过氧化速率和膜疏水性显著降低。在-3--5℃下储存24小时并随后解冻导致微粒体迅速聚集。
