一种用于估算红细胞葡萄糖磷酸异构酶的改良筛查方法。

Can J Comp Med. 1974 Jan;38(1):82-4.

A fluorimetric method to estimate erythrocyte glucose phosphate isomerase is described. Five mul of blood is added to 100 mul of water. Ten mul of the resulting hemolysate is incubated with a reaction mixture (200 mul) containing Tris-HCl buffer pH 8.0, 20 mumoles, MgCl(2) 2 mumoles, nicotinamide adenine dinucleotide phosphate 0.08 mumoles, glucose-6-phosphate dehydrogenase 0.2 EU and fructose-6-phosphate 0.12 mumoles. After ten minutes of 25 degrees C 20 mul of the mixture is added to 2 ml of 0.01 M phosphate buffer pH 7.4 and the nicotinamide adenine dinucleotide phosphate fluorescence determined. Two ml of water was added to the remaining reaction mixture and the hemoglobin concentration determined at 410 nm. The technique is primarily designed for use with small amounts of blood, of widely varying activity, from various animal species.

本文描述了一种用于估算红细胞葡萄糖磷酸异构酶的荧光法。将5微升血液加入到100微升水中。取10微升所得溶血产物与含有pH 8.0的Tris-HCl缓冲液20微摩尔、氯化镁2微摩尔、烟酰胺腺嘌呤二核苷酸磷酸0.08微摩尔、葡萄糖-6-磷酸脱氢酶0.2酶活力单位和果糖-6-磷酸0.12微摩尔的反应混合物（200微升）一起孵育。在25℃孵育10分钟后，取20微升混合物加入到2毫升pH 7.4的0.01 M磷酸盐缓冲液中，并测定烟酰胺腺嘌呤二核苷酸磷酸荧光。向剩余反应混合物中加入2毫升水，并在410纳米处测定血红蛋白浓度。该技术主要设计用于处理来自各种动物物种的少量血液，这些血液的活性差异很大。