布氏锥虫DNA聚合酶的检测与特性分析

Detection and characterization of DNA polymerase from Trypanosoma brucei.

作者信息

Dube D K, Williams R O, Seal G, Williams S C

出版信息

Biochim Biophys Acta. 1979 Jan 26;561(1):10-6. doi: 10.1016/0005-2787(79)90485-4.

PMID:420846

Abstract

The predominant DNA polymerase activity has been isolated from the parasitic flagellated protozoan, Trypanosoma brucei. Like mammalian DNA polymerase-alpha the trypanosome DNA polymerase is of large molecular weight (S, 6--8), is resistant to thermal denaturation, is sensitive to N-ethylmaleimide, and is inhibited by high ionic strength. However, specific antisera that cross-react with mammalian DNA polymerase-alpha from different species fail to cross-react with the trypanosome polymerase.

摘要

主要的DNA聚合酶活性已从寄生性鞭毛虫原生动物布氏锥虫中分离出来。与哺乳动物的DNA聚合酶α一样，锥虫的DNA聚合酶分子量很大（S值为6 - 8），对热变性有抗性，对N - 乙基马来酰亚胺敏感，并受到高离子强度的抑制。然而，能与不同物种的哺乳动物DNA聚合酶α发生交叉反应的特异性抗血清却不能与锥虫聚合酶发生交叉反应。