Emborg C
Appl Microbiol. 1974 May;27(5):830-3. doi: 10.1128/am.27.5.830-833.1974.
Dried preparations with Streptococcus faecium, strain A(2)1, and spores of Bacillus sphaericus, strain C(I)A, normally used for control of the microbiological efficiency of radiation sterilization plants and preparations with spores of Bacillus subtilis, normally used for control of sterilization by dry heat, formalin, and ethylene oxide, as well as similar preparations with Micrococcus radiodurans, strain R(1), and spores of Bacillus globigii (B. subtilis, var. niger) were gamma irradiated with dose rates from 16 to 70 krad/h at temperatures from 60 to 100 C. At 80 C the radiation response of the spore preparations was the same as at room temperature, whereas the radiation resistance of the preparations with the two vegetative strains was reduced. At 100 C the radiation response of preparations with spores of B. subtilis was unaffected by the high temperature, whereas at 16 and and 25 krad/h the radiation resistance of the radiation-resistant sporeformer B. sphaericus, strain C(I)A, was reduced to the level of radiation resistance of preparations with spores of B. subtilis. It is concluded that combinations of heat and gamma irradiation at the temperatures and dose rates tested may have very few practical applications in sterilization of medical equipment.
含有粪肠球菌A(2)1菌株的干燥制剂、球形芽孢杆菌C(I)A菌株的孢子，通常用于控制辐射灭菌工厂的微生物效率；含有枯草芽孢杆菌孢子的制剂，通常用于控制干热、福尔马林和环氧乙烷灭菌；以及含有耐辐射微球菌R(1)菌株和球形芽孢杆菌（枯草芽孢杆菌，黑色变种）孢子的类似制剂，在60至100℃的温度下，以16至70krad/h的剂量率进行γ射线辐照。在80℃时，孢子制剂的辐射响应与室温下相同，而含有两种营养菌株的制剂的辐射抗性降低。在100℃时，枯草芽孢杆菌孢子制剂的辐射响应不受高温影响，而在16和25krad/h时，耐辐射芽孢形成菌球形芽孢杆菌C(I)A菌株的辐射抗性降低到含有枯草芽孢杆菌孢子制剂的辐射抗性水平。得出的结论是，在所测试的温度和剂量率下，热和γ射线辐照的组合在医疗设备灭菌中可能几乎没有实际应用。
