Sandok P L, Hinsdill R D, Albrecht R M
Infect Immun. 1974 Jun;9(6):1045-50. doi: 10.1128/iai.9.6.1045-1050.1974.
Cultures of brucella-sensitized mouse spleen cells exposed to Brucella abortus antigens in vitro release macrophage migration inhibition factor (MIF) and macrophage spreading factor. Subjecting the supernatants from such cultures to preparative scale electrophoresis in acrylamide gel yields several fractions, one of which contains both MIF and macrophage spreading factor. This material has properties attributable to guinea pig MIF: it is nondialyzable, heat stable, nontoxic to macrophages from heterologous murine donors, and has a greater anodal electrophoretic mobility than guinea pig serum albumin. Another fraction from the gel column inhibits macrophage spreading; its electrophoretic mobility is similar to that of guinea pig gamma globulin. Neither brucella antigen nor skin reactive substances were detectable in any acrylamide gel column fraction when tested by the mouse footpad induration assay technique.
体外将布鲁氏菌致敏的小鼠脾细胞与流产布鲁氏菌抗原接触进行培养,可释放巨噬细胞移动抑制因子(MIF)和巨噬细胞扩散因子。将此类培养物的上清液进行聚丙烯酰胺凝胶制备规模电泳,可得到几个组分,其中一个组分同时含有MIF和巨噬细胞扩散因子。该物质具有豚鼠MIF的特性:不可透析、热稳定、对来自异种小鼠供体的巨噬细胞无毒,且在阳极电泳中的迁移率比豚鼠血清白蛋白高。凝胶柱的另一个组分可抑制巨噬细胞扩散;其电泳迁移率与豚鼠γ球蛋白相似。当通过小鼠足垫硬结试验技术检测时,在任何聚丙烯酰胺凝胶柱组分中均未检测到布鲁氏菌抗原或皮肤反应性物质。