一种用于测量循环免疫复合物的简单放射性标记类风湿因子结合试验。
A simple radiolabelled rheumatoid factor binding assay for the measurement of circulating immune complexes.
作者信息
Barratt J, Naish R
出版信息
J Immunol Methods. 1979;25(2):137-46. doi: 10.1016/0022-1759(79)90049-8.
A simple assay for circulating immune complexes has been developed and the optimal conditions for reaction defined. Partially purified radiolabelled polyclonal or monoclonal rheumatoid factor was incubated with aggregated IgG or soluble immune complexes and the resultant macromolecule precipitated with 3% polyethylene glycol. Monoclonal rheumatoid factor was much the better reagent, allowing the detection of approx. 0.35microgram/ml or more of aggregated IgG. Soluble immune complexes formed in vitro at between x2--x20 antigen excess were detectable over a wide range of molecular size. Clinical studies indicated that the assay is a useful addition to the currently available techniques for measuring circulating immune complexes.
已开发出一种用于检测循环免疫复合物的简单检测方法，并确定了反应的最佳条件。将部分纯化的放射性标记多克隆或单克隆类风湿因子与聚集的IgG或可溶性免疫复合物一起孵育，然后用3%聚乙二醇沉淀所得大分子。单克隆类风湿因子是更好的试剂，能够检测出约0.35微克/毫升或更多的聚集IgG。在体外抗原过量2至20倍之间形成的可溶性免疫复合物在很宽的分子大小范围内均可检测到。临床研究表明，该检测方法是对目前可用的循环免疫复合物检测技术的有益补充。
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