[Analysis of soluble antigens of the human striated musculature. I. Electrophoretic analysis and enzyme determinations].

Human skeletal muscle was extracted at pH 7. The supernatant of centrifugation at 100 000 X g for 1 h was analysed with different elektrophoretical methods and examined for activities of nine enzymes. These studies revealed an unique pattern of proteins in muscle extracts in comparison to human serum and differences of enzyme activities in both fluids. The bulk of muscle proteins migrated electrophoretically in the region of beta-and gamma-globulins. At least 10 substances or groups of substances could be detected in 100 000 X g supernatants of muscle extracts. The results are presuppositions for further analytical studies of muscle proteins with immunochemical methods.