Mohajer S, Gabliks J
J Exp Med. 1966 Jan 1;123(1):17-24. doi: 10.1084/jem.123.1.17.
The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10(-3) and 4.0 x 10(-3) moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.
通过使用甲硫氨酸类似物L-乙硫氨酸，研究了甲硫氨酸在脊髓灰质炎病毒感染HeLa细胞和猴肾细胞中的作用。在存在2.0×10⁻³和4.0×10⁻³摩尔乙硫氨酸的情况下，HeLa细胞和猴肾细胞的生长受到显著抑制。在相同实验条件下，乙硫氨酸对HeLa细胞中两株脊髓灰质炎病毒（Mahoney株和Lansing株）的生物合成没有显著影响，而在原代猴肾细胞中，它显著抑制了脊髓灰质炎病毒Lansing株的生物合成。细胞内氨基酸部分耗尽的HeLa细胞并未改变病毒生物合成的速率。添加过量的L-甲硫氨酸可逆转乙硫氨酸对细胞生长和病毒生物合成的抑制作用。
