Ward J B, Perkins H R
Biochem J. 1968 Jan;106(2):391-400. doi: 10.1042/bj1060391.
Membrane fractions were prepared from Staphylococcus aureus H and 100 after dissolution of the cell walls by a lytic enzyme from Streptomyces griseus. Membranes were also prepared from the L-forms derived from the same strains. The membranes were analysed for protein, lipid, carbohydrate and RNA contents, and the fatty acid composition of the lipids was determined. A branched-chain saturated C(15) acid was the major component in all samples, and the correspondence between L-forms and parent bacteria was fairly close. The lipids were separated into non-polar-lipid, glycolipid and phospholipid fractions; the L-forms contained a little more neutral lipid and much more glycolipid than the parent bacteria. In all membranes the glycolipid, which accounted for all the carbohydrate present, was a diglucosyl diglyceride. The major phospholipids of the protoplast membranes were phosphatidylglycerol and some lipoamino acids (lysine and a little alanine). On the other hand, diphosphatidylglycerol was the chief phospholipid found in L-form membranes.
用灰色链霉菌的一种裂解酶溶解细胞壁后，从金黄色葡萄球菌H和100制备膜组分。也从相同菌株衍生的L型菌制备膜。分析膜的蛋白质、脂质、碳水化合物和RNA含量，并测定脂质的脂肪酸组成。支链饱和C(15)酸是所有样品中的主要成分，L型菌与亲代细菌之间的对应关系相当密切。脂质被分离为非极性脂质、糖脂和磷脂组分；L型菌比亲代细菌含有更多一点的中性脂质和更多的糖脂。在所有膜中，占所有存在的碳水化合物的糖脂是二葡糖基二甘油酯。原生质体膜的主要磷脂是磷脂酰甘油和一些脂氨基酸（赖氨酸和少量丙氨酸）。另一方面，二磷脂酰甘油是在L型菌膜中发现的主要磷脂。
