Ginsel L A, Onderwater J J, Daems W T
Virchows Arch B Cell Pathol Incl Mol Pathol. 1979 Jun 29;30(3):245-73. doi: 10.1007/BF02889106.
The transport of 3H-fucose- and 3H-glucosamine-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue was investigated with light- and electron-microscopical autoradiography. The findings showed that these glycoproteins were completed in the Golgi apparatus and transported in small vesicular structures to the apical cytoplasm of these cells. Since this material arrived in the cell coat on the microvilli and in the lysosome-like bodies simultaneously, a crinophagic function of these organelles in the regulation of the transport or secretion of cell-coat material was supported. In the absorptive cells of patients with fucosidosis or Hunter's type of lysosomal storage disease, a smiliar transport of cell-coat material to the lysosome-like bodies and a congenital defect of a lysosomal hydrolase normally involved in the degradation of cell-coat material, can explain the accumulation of this material in the dense bodies.
采用光镜和电镜放射自显影技术，对培养的人小肠组织吸收细胞中3H-岩藻糖和3H-葡糖胺标记的糖蛋白转运进行了研究。结果表明，这些糖蛋白在高尔基体中合成，并通过小泡结构转运至这些细胞的顶端细胞质。由于这种物质同时到达微绒毛上的细胞衣和溶酶体样小体，因此支持了这些细胞器在调节细胞衣物质转运或分泌方面的噬分泌功能。在岩藻糖苷贮积症或亨特型溶酶体贮积病患者的吸收细胞中，细胞衣物质向溶酶体样小体的类似转运以及通常参与细胞衣物质降解的溶酶体水解酶的先天性缺陷，可以解释这种物质在致密小体中的积累。
