大鼠小肠锌吸收部位的测定。

Determination of the site of zinc absorption in rat small intestine.

作者信息

Antonson D L, Barak A J, Vanderhoof J A

出版信息

J Nutr. 1979 Jan;109(1):142-7. doi: 10.1093/jn/109.1.142.

DOI:10.1093/jn/109.1.142

Abstract

We have studied net uptake of zinc from segments of rat duodenum, jejunum, and ileum using in vivo intestinal perfusion. We have also evaluated the effect of pancreatic and bile secretions on duodenal zinc absorption. Segments of duodenum, jejunum, and ileum and duodenum with bile and pancreatic duct obstruction, 10 cm in length, were studied in six rats each. Percentage absorption of zinc as determined by atomic absorption spectrophotomety was greater from the ileum (60.1%) when compared to duodenum (19.1%) or jejunum (20.2%). Exclusion of bile and pancreatic secretions from the lumen increased zinc absorption in the duodenum (32.0%). Postperfusion mucosal zinc content comprised 29.0% of absorbed zinc from jejunum, 7.4% from ileum, 5.2% from duodenum, and 2.7% from duodenum with bile and pancreatic duct obstructed, indicating rapid transport of zinc across epithelial cells after uptake from the duodenum and ileum. Our data indicate that the ileum has the greatest capacity for zinc absorption.

摘要

我们利用体内肠道灌注法研究了大鼠十二指肠、空肠和回肠段对锌的净摄取。我们还评估了胰液和胆汁分泌对十二指肠锌吸收的影响。分别对十二指肠、空肠和回肠段以及胆管和胰管梗阻的十二指肠段（长度均为10厘米）进行了研究，每组6只大鼠。通过原子吸收分光光度法测定，回肠的锌吸收百分比（60.1%）高于十二指肠（19.1%）或空肠（20.2%）。肠腔内排除胆汁和胰液分泌可增加十二指肠的锌吸收（32.0%）。灌注后，空肠黏膜锌含量占吸收锌的29.0%，回肠为7.4%，十二指肠为5.2%，胆管和胰管梗阻的十二指肠为2.7%，这表明锌从十二指肠和回肠摄取后迅速穿过上皮细胞进行转运。我们的数据表明，回肠具有最大的锌吸收能力。