Grand R J, Gross P R
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1081-8. doi: 10.1073/pnas.65.4.1081.
The synthesis of RNA, protein, and amylase has been studied in normal and epinephrine-treated slices of rat parotid gland. Epinephrine stimulates synthesis of total cell protein and specifically of amylase, but amylase synthesis is preferentially increased. There is no change in either the specific radioactivity or pattern of labeling of total RNA. In the presence of actinomycin D, epinephrine stimulates secretion of amylase, as well as protein and amylase synthesis. RNA synthesis under these conditions is reduced to 10 per cent of control values. Slices obtained from animals treated with actinomycin D nine and a half hours prior to study show responses to epinephrine comparable to those from control animals, with RNA synthesis inhibited by 70 per cent. Since amylase synthesis is stimulated by epinephrine in the absence of RNA synthesis, control of the production of this enzyme is probably exerted at the level of translation.
在正常和肾上腺素处理的大鼠腮腺切片中，对RNA、蛋白质和淀粉酶的合成进行了研究。肾上腺素刺激总细胞蛋白质的合成，特别是淀粉酶的合成，但淀粉酶合成优先增加。总RNA的比放射性或标记模式均无变化。在放线菌素D存在的情况下，肾上腺素刺激淀粉酶的分泌以及蛋白质和淀粉酶的合成。在这些条件下，RNA合成降至对照值的10%。在研究前9个半小时用放线菌素D处理的动物所获得的切片对肾上腺素的反应与对照动物的反应相当，RNA合成被抑制70%。由于在没有RNA合成的情况下，肾上腺素刺激淀粉酶的合成，这种酶产生的控制可能在翻译水平上发挥作用。
