Chapman A G, Fall L, Atkinson D E
J Bacteriol. 1971 Dec;108(3):1072-86. doi: 10.1128/jb.108.3.1072-1086.1971.
The value of the adenylate energy charge, [(adenosine triphosphate) + (1/2) (adenosine diphosphate)]/[(adenosine triphosphate) + (adenosine diphosphate) + (adenosine monophosphate)], in Escherichia coli cells during growth is about 0.8. During the stationary phase after cessation of growth, or during starvation in carbon-limited cultures, the energy charge declines slowly to a value of about 0.5, and then falls more rapidly. During the slow decline in energy charge, all the cells are capable of forming colonies, but a rapid fall in viability coincides with the steep drop in energy charge. These results suggest that growth can occur only at energy charge values above about 0.8, that viability is maintained at values between 0.8 and 0.5, and that cells die at values below 0.5. Tabulation of adenylate concentrations previously reported for various organisms and tissues supports the prediction, based on enzyme kinetic observations in vitro, that the energy charge is stabilized near 0.85 in intact metabolizing cells of a wide variety of types.
在大肠杆菌细胞生长过程中，腺苷酸能荷，即[（三磷酸腺苷）+（1/2）（二磷酸腺苷）]/[（三磷酸腺苷）+（二磷酸腺苷）+（一磷酸腺苷）]的值约为0.8。在生长停止后的稳定期，或在碳源受限培养物中的饥饿期间，能荷缓慢下降至约0.5的值，然后下降得更快。在能荷缓慢下降期间，所有细胞都能够形成菌落，但活力的快速下降与能荷的急剧下降同时发生。这些结果表明，生长仅能在能荷值高于约0.8时发生，活力在0.8至0.5的值之间维持，而细胞在低于0.5的值时死亡。先前报道的各种生物体和组织的腺苷酸浓度列表支持了基于体外酶动力学观察的预测，即在各种类型的完整代谢细胞中，能荷稳定在0.85附近。
