Blair C D, Brennan P J
J Virol. 1972 May;9(5):813-22. doi: 10.1128/JVI.9.5.813-822.1972.
Lipid metabolism in the "abortive" system, Sendai virus-infected primary chick embryo fibroblasts, was examined by using (32)P-orthophosphate, (14)C-glucose, and (14)C-glycerol as precursors. Incorporation of radioactivity from (32)P-orthophosphate and (14)C-glucose into lipid was increased in infected cells. Synthesis of all individual phospholipids was about equally stimulated. There was also evidence for increased lipid synthesis in more productively infected monkey kidney cells. Incorporation of (14)C-glycerol when at a high level in the medium was also increased. However, when this precursor was supplied in minute quantities of high specific activity, incorporation was inhibited. Even though incorporation of radioactivity from (14)C-glucose was stimulated during long labeling periods, the uptake of this precursor during short pulses was inhibited in infected cells. The phenomenon of increased labeling of triglyceride in infected chick cells under certain conditions is discussed, in conjunction with the other effects, in terms of related changes in other virus-infected systems.
利用（32）P-正磷酸盐、（14）C-葡萄糖和（14）C-甘油作为前体，对仙台病毒感染的原代鸡胚成纤维细胞这一“流产”系统中的脂质代谢进行了研究。感染细胞中，（32）P-正磷酸盐和（14）C-葡萄糖的放射性掺入脂质的量增加。所有单个磷脂的合成受到的刺激程度大致相同。在感染效率更高的猴肾细胞中，也有脂质合成增加的证据。培养基中（14）C-甘油水平较高时，其掺入量也增加。然而，当以高比活性的微量形式提供这种前体时，掺入受到抑制。尽管在长时间标记期间，（14）C-葡萄糖的放射性掺入受到刺激，但在短脉冲期间，感染细胞对这种前体的摄取受到抑制。结合其他病毒感染系统中的相关变化，讨论了在某些条件下感染鸡细胞中甘油三酯标记增加的现象以及其他影响。
