Drabikowska A K, Kruszewska A
J Bacteriol. 1972 Dec;112(3):1112-7. doi: 10.1128/jb.112.3.1112-1117.1972.
Mitochondria of cytoplasmic respiratory mutants [mi-1] (poky) and [mi-4] contain about a fourfold molar excess of ubiquinone as compared to the wild-type strain of Neurospora crassa. In the wild type and [mi-1] cultures the concentration of ubiquinone remains constant during the exponential and stationary phase of growth. In [mi-4] cultures it markedly decreases in the stationary phase. The reduction of ubiquinone by substrates is approximately the same in the three strains tested and amounts 60 to 70% of total ubiquinone present in mitochondria, independent of its absolute amount. The reduction of ubiquinone on addition of substrates is accompanied by the similar reduction of cytochrome c. These indicate that mitochondrial ubiquinone and cytochrome c are involved in processes of oxidation in Neurospora and that ubiquinone belongs mainly if not entirely to the cytochrome system of electron transport in these strains.
细胞质呼吸突变体[mi - 1]（迟缓型）和[mi - 4]的线粒体与野生型粗糙脉孢菌相比，泛醌的摩尔过量约为四倍。在野生型和[mi - 1]培养物中，泛醌的浓度在生长的指数期和稳定期保持恒定。在[mi - 4]培养物中，它在稳定期显著降低。在所测试的三种菌株中，底物对泛醌的还原作用大致相同，占线粒体中总泛醌的60%至70%，与泛醌的绝对量无关。添加底物时泛醌的还原伴随着细胞色素c的类似还原。这些表明线粒体泛醌和细胞色素c参与了粗糙脉孢菌的氧化过程，并且泛醌在这些菌株中如果不完全属于电子传递的细胞色素系统，也主要属于该系统。
